Educational resources and simple solutions for your research journey

Research hypothesis: What it is, how to write it, types, and examples

What is a Research Hypothesis: How to Write it, Types, and Examples

formulation of hypothesis examples

Any research begins with a research question and a research hypothesis . A research question alone may not suffice to design the experiment(s) needed to answer it. A hypothesis is central to the scientific method. But what is a hypothesis ? A hypothesis is a testable statement that proposes a possible explanation to a phenomenon, and it may include a prediction. Next, you may ask what is a research hypothesis ? Simply put, a research hypothesis is a prediction or educated guess about the relationship between the variables that you want to investigate.  

It is important to be thorough when developing your research hypothesis. Shortcomings in the framing of a hypothesis can affect the study design and the results. A better understanding of the research hypothesis definition and characteristics of a good hypothesis will make it easier for you to develop your own hypothesis for your research. Let’s dive in to know more about the types of research hypothesis , how to write a research hypothesis , and some research hypothesis examples .  

Table of Contents

What is a hypothesis ?  

A hypothesis is based on the existing body of knowledge in a study area. Framed before the data are collected, a hypothesis states the tentative relationship between independent and dependent variables, along with a prediction of the outcome.  

What is a research hypothesis ?  

Young researchers starting out their journey are usually brimming with questions like “ What is a hypothesis ?” “ What is a research hypothesis ?” “How can I write a good research hypothesis ?”   

A research hypothesis is a statement that proposes a possible explanation for an observable phenomenon or pattern. It guides the direction of a study and predicts the outcome of the investigation. A research hypothesis is testable, i.e., it can be supported or disproven through experimentation or observation.     

formulation of hypothesis examples

Characteristics of a good hypothesis  

Here are the characteristics of a good hypothesis :  

  • Clearly formulated and free of language errors and ambiguity  
  • Concise and not unnecessarily verbose  
  • Has clearly defined variables  
  • Testable and stated in a way that allows for it to be disproven  
  • Can be tested using a research design that is feasible, ethical, and practical   
  • Specific and relevant to the research problem  
  • Rooted in a thorough literature search  
  • Can generate new knowledge or understanding.  

How to create an effective research hypothesis  

A study begins with the formulation of a research question. A researcher then performs background research. This background information forms the basis for building a good research hypothesis . The researcher then performs experiments, collects, and analyzes the data, interprets the findings, and ultimately, determines if the findings support or negate the original hypothesis.  

Let’s look at each step for creating an effective, testable, and good research hypothesis :  

  • Identify a research problem or question: Start by identifying a specific research problem.   
  • Review the literature: Conduct an in-depth review of the existing literature related to the research problem to grasp the current knowledge and gaps in the field.   
  • Formulate a clear and testable hypothesis : Based on the research question, use existing knowledge to form a clear and testable hypothesis . The hypothesis should state a predicted relationship between two or more variables that can be measured and manipulated. Improve the original draft till it is clear and meaningful.  
  • State the null hypothesis: The null hypothesis is a statement that there is no relationship between the variables you are studying.   
  • Define the population and sample: Clearly define the population you are studying and the sample you will be using for your research.  
  • Select appropriate methods for testing the hypothesis: Select appropriate research methods, such as experiments, surveys, or observational studies, which will allow you to test your research hypothesis .  

Remember that creating a research hypothesis is an iterative process, i.e., you might have to revise it based on the data you collect. You may need to test and reject several hypotheses before answering the research problem.  

How to write a research hypothesis  

When you start writing a research hypothesis , you use an “if–then” statement format, which states the predicted relationship between two or more variables. Clearly identify the independent variables (the variables being changed) and the dependent variables (the variables being measured), as well as the population you are studying. Review and revise your hypothesis as needed.  

An example of a research hypothesis in this format is as follows:  

“ If [athletes] follow [cold water showers daily], then their [endurance] increases.”  

Population: athletes  

Independent variable: daily cold water showers  

Dependent variable: endurance  

You may have understood the characteristics of a good hypothesis . But note that a research hypothesis is not always confirmed; a researcher should be prepared to accept or reject the hypothesis based on the study findings.  

formulation of hypothesis examples

Research hypothesis checklist  

Following from above, here is a 10-point checklist for a good research hypothesis :  

  • Testable: A research hypothesis should be able to be tested via experimentation or observation.  
  • Specific: A research hypothesis should clearly state the relationship between the variables being studied.  
  • Based on prior research: A research hypothesis should be based on existing knowledge and previous research in the field.  
  • Falsifiable: A research hypothesis should be able to be disproven through testing.  
  • Clear and concise: A research hypothesis should be stated in a clear and concise manner.  
  • Logical: A research hypothesis should be logical and consistent with current understanding of the subject.  
  • Relevant: A research hypothesis should be relevant to the research question and objectives.  
  • Feasible: A research hypothesis should be feasible to test within the scope of the study.  
  • Reflects the population: A research hypothesis should consider the population or sample being studied.  
  • Uncomplicated: A good research hypothesis is written in a way that is easy for the target audience to understand.  

By following this research hypothesis checklist , you will be able to create a research hypothesis that is strong, well-constructed, and more likely to yield meaningful results.  

Research hypothesis: What it is, how to write it, types, and examples

Types of research hypothesis  

Different types of research hypothesis are used in scientific research:  

1. Null hypothesis:

A null hypothesis states that there is no change in the dependent variable due to changes to the independent variable. This means that the results are due to chance and are not significant. A null hypothesis is denoted as H0 and is stated as the opposite of what the alternative hypothesis states.   

Example: “ The newly identified virus is not zoonotic .”  

2. Alternative hypothesis:

This states that there is a significant difference or relationship between the variables being studied. It is denoted as H1 or Ha and is usually accepted or rejected in favor of the null hypothesis.  

Example: “ The newly identified virus is zoonotic .”  

3. Directional hypothesis :

This specifies the direction of the relationship or difference between variables; therefore, it tends to use terms like increase, decrease, positive, negative, more, or less.   

Example: “ The inclusion of intervention X decreases infant mortality compared to the original treatment .”   

4. Non-directional hypothesis:

While it does not predict the exact direction or nature of the relationship between the two variables, a non-directional hypothesis states the existence of a relationship or difference between variables but not the direction, nature, or magnitude of the relationship. A non-directional hypothesis may be used when there is no underlying theory or when findings contradict previous research.  

Example, “ Cats and dogs differ in the amount of affection they express .”  

5. Simple hypothesis :

A simple hypothesis only predicts the relationship between one independent and another independent variable.  

Example: “ Applying sunscreen every day slows skin aging .”  

6 . Complex hypothesis :

A complex hypothesis states the relationship or difference between two or more independent and dependent variables.   

Example: “ Applying sunscreen every day slows skin aging, reduces sun burn, and reduces the chances of skin cancer .” (Here, the three dependent variables are slowing skin aging, reducing sun burn, and reducing the chances of skin cancer.)  

7. Associative hypothesis:  

An associative hypothesis states that a change in one variable results in the change of the other variable. The associative hypothesis defines interdependency between variables.  

Example: “ There is a positive association between physical activity levels and overall health .”  

8 . Causal hypothesis:

A causal hypothesis proposes a cause-and-effect interaction between variables.  

Example: “ Long-term alcohol use causes liver damage .”  

Note that some of the types of research hypothesis mentioned above might overlap. The types of hypothesis chosen will depend on the research question and the objective of the study.  

formulation of hypothesis examples

Research hypothesis examples  

Here are some good research hypothesis examples :  

“The use of a specific type of therapy will lead to a reduction in symptoms of depression in individuals with a history of major depressive disorder.”  

“Providing educational interventions on healthy eating habits will result in weight loss in overweight individuals.”  

“Plants that are exposed to certain types of music will grow taller than those that are not exposed to music.”  

“The use of the plant growth regulator X will lead to an increase in the number of flowers produced by plants.”  

Characteristics that make a research hypothesis weak are unclear variables, unoriginality, being too general or too vague, and being untestable. A weak hypothesis leads to weak research and improper methods.   

Some bad research hypothesis examples (and the reasons why they are “bad”) are as follows:  

“This study will show that treatment X is better than any other treatment . ” (This statement is not testable, too broad, and does not consider other treatments that may be effective.)  

“This study will prove that this type of therapy is effective for all mental disorders . ” (This statement is too broad and not testable as mental disorders are complex and different disorders may respond differently to different types of therapy.)  

“Plants can communicate with each other through telepathy . ” (This statement is not testable and lacks a scientific basis.)  

Importance of testable hypothesis  

If a research hypothesis is not testable, the results will not prove or disprove anything meaningful. The conclusions will be vague at best. A testable hypothesis helps a researcher focus on the study outcome and understand the implication of the question and the different variables involved. A testable hypothesis helps a researcher make precise predictions based on prior research.  

To be considered testable, there must be a way to prove that the hypothesis is true or false; further, the results of the hypothesis must be reproducible.  

Research hypothesis: What it is, how to write it, types, and examples

Frequently Asked Questions (FAQs) on research hypothesis  

1. What is the difference between research question and research hypothesis ?  

A research question defines the problem and helps outline the study objective(s). It is an open-ended statement that is exploratory or probing in nature. Therefore, it does not make predictions or assumptions. It helps a researcher identify what information to collect. A research hypothesis , however, is a specific, testable prediction about the relationship between variables. Accordingly, it guides the study design and data analysis approach.

2. When to reject null hypothesis ?

A null hypothesis should be rejected when the evidence from a statistical test shows that it is unlikely to be true. This happens when the test statistic (e.g., p -value) is less than the defined significance level (e.g., 0.05). Rejecting the null hypothesis does not necessarily mean that the alternative hypothesis is true; it simply means that the evidence found is not compatible with the null hypothesis.  

3. How can I be sure my hypothesis is testable?  

A testable hypothesis should be specific and measurable, and it should state a clear relationship between variables that can be tested with data. To ensure that your hypothesis is testable, consider the following:  

  • Clearly define the key variables in your hypothesis. You should be able to measure and manipulate these variables in a way that allows you to test the hypothesis.  
  • The hypothesis should predict a specific outcome or relationship between variables that can be measured or quantified.   
  • You should be able to collect the necessary data within the constraints of your study.  
  • It should be possible for other researchers to replicate your study, using the same methods and variables.   
  • Your hypothesis should be testable by using appropriate statistical analysis techniques, so you can draw conclusions, and make inferences about the population from the sample data.  
  • The hypothesis should be able to be disproven or rejected through the collection of data.  

4. How do I revise my research hypothesis if my data does not support it?  

If your data does not support your research hypothesis , you will need to revise it or develop a new one. You should examine your data carefully and identify any patterns or anomalies, re-examine your research question, and/or revisit your theory to look for any alternative explanations for your results. Based on your review of the data, literature, and theories, modify your research hypothesis to better align it with the results you obtained. Use your revised hypothesis to guide your research design and data collection. It is important to remain objective throughout the process.  

5. I am performing exploratory research. Do I need to formulate a research hypothesis?  

As opposed to “confirmatory” research, where a researcher has some idea about the relationship between the variables under investigation, exploratory research (or hypothesis-generating research) looks into a completely new topic about which limited information is available. Therefore, the researcher will not have any prior hypotheses. In such cases, a researcher will need to develop a post-hoc hypothesis. A post-hoc research hypothesis is generated after these results are known.  

6. How is a research hypothesis different from a research question?

A research question is an inquiry about a specific topic or phenomenon, typically expressed as a question. It seeks to explore and understand a particular aspect of the research subject. In contrast, a research hypothesis is a specific statement or prediction that suggests an expected relationship between variables. It is formulated based on existing knowledge or theories and guides the research design and data analysis.

7. Can a research hypothesis change during the research process?

Yes, research hypotheses can change during the research process. As researchers collect and analyze data, new insights and information may emerge that require modification or refinement of the initial hypotheses. This can be due to unexpected findings, limitations in the original hypotheses, or the need to explore additional dimensions of the research topic. Flexibility is crucial in research, allowing for adaptation and adjustment of hypotheses to align with the evolving understanding of the subject matter.

8. How many hypotheses should be included in a research study?

The number of research hypotheses in a research study varies depending on the nature and scope of the research. It is not necessary to have multiple hypotheses in every study. Some studies may have only one primary hypothesis, while others may have several related hypotheses. The number of hypotheses should be determined based on the research objectives, research questions, and the complexity of the research topic. It is important to ensure that the hypotheses are focused, testable, and directly related to the research aims.

9. Can research hypotheses be used in qualitative research?

Yes, research hypotheses can be used in qualitative research, although they are more commonly associated with quantitative research. In qualitative research, hypotheses may be formulated as tentative or exploratory statements that guide the investigation. Instead of testing hypotheses through statistical analysis, qualitative researchers may use the hypotheses to guide data collection and analysis, seeking to uncover patterns, themes, or relationships within the qualitative data. The emphasis in qualitative research is often on generating insights and understanding rather than confirming or rejecting specific research hypotheses through statistical testing.

Researcher.Life is a subscription-based platform that unifies the best AI tools and services designed to speed up, simplify, and streamline every step of a researcher’s journey. The Researcher.Life All Access Pack is a one-of-a-kind subscription that unlocks full access to an AI writing assistant, literature recommender, journal finder, scientific illustration tool, and exclusive discounts on professional publication services from Editage.  

Based on 21+ years of experience in academia, Researcher.Life All Access empowers researchers to put their best research forward and move closer to success. Explore our top AI Tools pack, AI Tools + Publication Services pack, or Build Your Own Plan. Find everything a researcher needs to succeed, all in one place –  Get All Access now starting at just $17 a month !    

Related Posts

case study in research

What is a Case Study in Research? Definition, Methods, and Examples

formulation of hypothesis examples

Take Top AI Tools for Researchers for a Spin with the Editage All Access 7-Day Pass!

Have a language expert improve your writing

Run a free plagiarism check in 10 minutes, automatically generate references for free.

  • Knowledge Base
  • Methodology
  • How to Write a Strong Hypothesis | Guide & Examples

How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

Prevent plagiarism, run a free check.

Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

  • The relevant variables
  • The specific group being studied
  • The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Research question Hypothesis Null hypothesis
What are the health benefits of eating an apple a day? Increasing apple consumption in over-60s will result in decreasing frequency of doctor’s visits. Increasing apple consumption in over-60s will have no effect on frequency of doctor’s visits.
Which airlines have the most delays? Low-cost airlines are more likely to have delays than premium airlines. Low-cost and premium airlines are equally likely to have delays.
Can flexible work arrangements improve job satisfaction? Employees who have flexible working hours will report greater job satisfaction than employees who work fixed hours. There is no relationship between working hour flexibility and job satisfaction.
How effective is secondary school sex education at reducing teen pregnancies? Teenagers who received sex education lessons throughout secondary school will have lower rates of unplanned pregnancy than teenagers who did not receive any sex education. Secondary school sex education has no effect on teen pregnancy rates.
What effect does daily use of social media have on the attention span of under-16s? There is a negative correlation between time spent on social media and attention span in under-16s. There is no relationship between social media use and attention span in under-16s.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

Cite this Scribbr article

If you want to cite this source, you can copy and paste the citation or click the ‘Cite this Scribbr article’ button to automatically add the citation to our free Reference Generator.

McCombes, S. (2022, May 06). How to Write a Strong Hypothesis | Guide & Examples. Scribbr. Retrieved 24 June 2024, from https://www.scribbr.co.uk/research-methods/hypothesis-writing/

Is this article helpful?

Shona McCombes

Shona McCombes

Other students also liked, operationalisation | a guide with examples, pros & cons, what is a conceptual framework | tips & examples, a quick guide to experimental design | 5 steps & examples.

  • Bipolar Disorder
  • Therapy Center
  • When To See a Therapist
  • Types of Therapy
  • Best Online Therapy
  • Best Couples Therapy
  • Best Family Therapy
  • Managing Stress
  • Sleep and Dreaming
  • Understanding Emotions
  • Self-Improvement
  • Healthy Relationships
  • Student Resources
  • Personality Types
  • Guided Meditations
  • Verywell Mind Insights
  • 2024 Verywell Mind 25
  • Mental Health in the Classroom
  • Editorial Process
  • Meet Our Review Board
  • Crisis Support

How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

formulation of hypothesis examples

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

formulation of hypothesis examples

Verywell / Alex Dos Diaz

  • The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

  • Operationalization

Hypothesis Types

Hypotheses examples.

  • Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

  • Forming a question
  • Performing background research
  • Creating a hypothesis
  • Designing an experiment
  • Collecting data
  • Analyzing the results
  • Drawing conclusions
  • Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

  • Is your hypothesis based on your research on a topic?
  • Can your hypothesis be tested?
  • Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

  • Collect as many observations about a topic or problem as you can.
  • Evaluate these observations and look for possible causes of the problem.
  • Create a list of possible explanations that you might want to explore.
  • After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

  • Does your hypothesis focus on something that you can actually test?
  • Does your hypothesis include both an independent and dependent variable?
  • Can you manipulate the variables?
  • Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

  • Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
  • Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
  • Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
  • Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
  • Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
  • Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

  • "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
  • "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
  • "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
  • "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

  • "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
  • "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

  • "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
  • "There is no difference in scores on a memory recall task between children and adults."
  • "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

  • "People who take St. John's wort supplements will have less anxiety than those who do not."
  • "Adults will perform better on a memory task than children."
  • "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

  • Resources Home 🏠
  • Try SciSpace Copilot
  • Search research papers
  • Add Copilot Extension
  • Try AI Detector
  • Try Paraphraser
  • Try Citation Generator
  • April Papers
  • June Papers
  • July Papers

SciSpace Resources

The Craft of Writing a Strong Hypothesis

Deeptanshu D

Table of Contents

Writing a hypothesis is one of the essential elements of a scientific research paper. It needs to be to the point, clearly communicating what your research is trying to accomplish. A blurry, drawn-out, or complexly-structured hypothesis can confuse your readers. Or worse, the editor and peer reviewers.

A captivating hypothesis is not too intricate. This blog will take you through the process so that, by the end of it, you have a better idea of how to convey your research paper's intent in just one sentence.

What is a Hypothesis?

The first step in your scientific endeavor, a hypothesis, is a strong, concise statement that forms the basis of your research. It is not the same as a thesis statement , which is a brief summary of your research paper .

The sole purpose of a hypothesis is to predict your paper's findings, data, and conclusion. It comes from a place of curiosity and intuition . When you write a hypothesis, you're essentially making an educated guess based on scientific prejudices and evidence, which is further proven or disproven through the scientific method.

The reason for undertaking research is to observe a specific phenomenon. A hypothesis, therefore, lays out what the said phenomenon is. And it does so through two variables, an independent and dependent variable.

The independent variable is the cause behind the observation, while the dependent variable is the effect of the cause. A good example of this is “mixing red and blue forms purple.” In this hypothesis, mixing red and blue is the independent variable as you're combining the two colors at your own will. The formation of purple is the dependent variable as, in this case, it is conditional to the independent variable.

Different Types of Hypotheses‌

Types-of-hypotheses

Types of hypotheses

Some would stand by the notion that there are only two types of hypotheses: a Null hypothesis and an Alternative hypothesis. While that may have some truth to it, it would be better to fully distinguish the most common forms as these terms come up so often, which might leave you out of context.

Apart from Null and Alternative, there are Complex, Simple, Directional, Non-Directional, Statistical, and Associative and casual hypotheses. They don't necessarily have to be exclusive, as one hypothesis can tick many boxes, but knowing the distinctions between them will make it easier for you to construct your own.

1. Null hypothesis

A null hypothesis proposes no relationship between two variables. Denoted by H 0 , it is a negative statement like “Attending physiotherapy sessions does not affect athletes' on-field performance.” Here, the author claims physiotherapy sessions have no effect on on-field performances. Even if there is, it's only a coincidence.

2. Alternative hypothesis

Considered to be the opposite of a null hypothesis, an alternative hypothesis is donated as H1 or Ha. It explicitly states that the dependent variable affects the independent variable. A good  alternative hypothesis example is “Attending physiotherapy sessions improves athletes' on-field performance.” or “Water evaporates at 100 °C. ” The alternative hypothesis further branches into directional and non-directional.

  • Directional hypothesis: A hypothesis that states the result would be either positive or negative is called directional hypothesis. It accompanies H1 with either the ‘<' or ‘>' sign.
  • Non-directional hypothesis: A non-directional hypothesis only claims an effect on the dependent variable. It does not clarify whether the result would be positive or negative. The sign for a non-directional hypothesis is ‘≠.'

3. Simple hypothesis

A simple hypothesis is a statement made to reflect the relation between exactly two variables. One independent and one dependent. Consider the example, “Smoking is a prominent cause of lung cancer." The dependent variable, lung cancer, is dependent on the independent variable, smoking.

4. Complex hypothesis

In contrast to a simple hypothesis, a complex hypothesis implies the relationship between multiple independent and dependent variables. For instance, “Individuals who eat more fruits tend to have higher immunity, lesser cholesterol, and high metabolism.” The independent variable is eating more fruits, while the dependent variables are higher immunity, lesser cholesterol, and high metabolism.

5. Associative and casual hypothesis

Associative and casual hypotheses don't exhibit how many variables there will be. They define the relationship between the variables. In an associative hypothesis, changing any one variable, dependent or independent, affects others. In a casual hypothesis, the independent variable directly affects the dependent.

6. Empirical hypothesis

Also referred to as the working hypothesis, an empirical hypothesis claims a theory's validation via experiments and observation. This way, the statement appears justifiable and different from a wild guess.

Say, the hypothesis is “Women who take iron tablets face a lesser risk of anemia than those who take vitamin B12.” This is an example of an empirical hypothesis where the researcher  the statement after assessing a group of women who take iron tablets and charting the findings.

7. Statistical hypothesis

The point of a statistical hypothesis is to test an already existing hypothesis by studying a population sample. Hypothesis like “44% of the Indian population belong in the age group of 22-27.” leverage evidence to prove or disprove a particular statement.

Characteristics of a Good Hypothesis

Writing a hypothesis is essential as it can make or break your research for you. That includes your chances of getting published in a journal. So when you're designing one, keep an eye out for these pointers:

  • A research hypothesis has to be simple yet clear to look justifiable enough.
  • It has to be testable — your research would be rendered pointless if too far-fetched into reality or limited by technology.
  • It has to be precise about the results —what you are trying to do and achieve through it should come out in your hypothesis.
  • A research hypothesis should be self-explanatory, leaving no doubt in the reader's mind.
  • If you are developing a relational hypothesis, you need to include the variables and establish an appropriate relationship among them.
  • A hypothesis must keep and reflect the scope for further investigations and experiments.

Separating a Hypothesis from a Prediction

Outside of academia, hypothesis and prediction are often used interchangeably. In research writing, this is not only confusing but also incorrect. And although a hypothesis and prediction are guesses at their core, there are many differences between them.

A hypothesis is an educated guess or even a testable prediction validated through research. It aims to analyze the gathered evidence and facts to define a relationship between variables and put forth a logical explanation behind the nature of events.

Predictions are assumptions or expected outcomes made without any backing evidence. They are more fictionally inclined regardless of where they originate from.

For this reason, a hypothesis holds much more weight than a prediction. It sticks to the scientific method rather than pure guesswork. "Planets revolve around the Sun." is an example of a hypothesis as it is previous knowledge and observed trends. Additionally, we can test it through the scientific method.

Whereas "COVID-19 will be eradicated by 2030." is a prediction. Even though it results from past trends, we can't prove or disprove it. So, the only way this gets validated is to wait and watch if COVID-19 cases end by 2030.

Finally, How to Write a Hypothesis

Quick-tips-on-how-to-write-a-hypothesis

Quick tips on writing a hypothesis

1.  Be clear about your research question

A hypothesis should instantly address the research question or the problem statement. To do so, you need to ask a question. Understand the constraints of your undertaken research topic and then formulate a simple and topic-centric problem. Only after that can you develop a hypothesis and further test for evidence.

2. Carry out a recce

Once you have your research's foundation laid out, it would be best to conduct preliminary research. Go through previous theories, academic papers, data, and experiments before you start curating your research hypothesis. It will give you an idea of your hypothesis's viability or originality.

Making use of references from relevant research papers helps draft a good research hypothesis. SciSpace Discover offers a repository of over 270 million research papers to browse through and gain a deeper understanding of related studies on a particular topic. Additionally, you can use SciSpace Copilot , your AI research assistant, for reading any lengthy research paper and getting a more summarized context of it. A hypothesis can be formed after evaluating many such summarized research papers. Copilot also offers explanations for theories and equations, explains paper in simplified version, allows you to highlight any text in the paper or clip math equations and tables and provides a deeper, clear understanding of what is being said. This can improve the hypothesis by helping you identify potential research gaps.

3. Create a 3-dimensional hypothesis

Variables are an essential part of any reasonable hypothesis. So, identify your independent and dependent variable(s) and form a correlation between them. The ideal way to do this is to write the hypothetical assumption in the ‘if-then' form. If you use this form, make sure that you state the predefined relationship between the variables.

In another way, you can choose to present your hypothesis as a comparison between two variables. Here, you must specify the difference you expect to observe in the results.

4. Write the first draft

Now that everything is in place, it's time to write your hypothesis. For starters, create the first draft. In this version, write what you expect to find from your research.

Clearly separate your independent and dependent variables and the link between them. Don't fixate on syntax at this stage. The goal is to ensure your hypothesis addresses the issue.

5. Proof your hypothesis

After preparing the first draft of your hypothesis, you need to inspect it thoroughly. It should tick all the boxes, like being concise, straightforward, relevant, and accurate. Your final hypothesis has to be well-structured as well.

Research projects are an exciting and crucial part of being a scholar. And once you have your research question, you need a great hypothesis to begin conducting research. Thus, knowing how to write a hypothesis is very important.

Now that you have a firmer grasp on what a good hypothesis constitutes, the different kinds there are, and what process to follow, you will find it much easier to write your hypothesis, which ultimately helps your research.

Now it's easier than ever to streamline your research workflow with SciSpace Discover . Its integrated, comprehensive end-to-end platform for research allows scholars to easily discover, write and publish their research and fosters collaboration.

It includes everything you need, including a repository of over 270 million research papers across disciplines, SEO-optimized summaries and public profiles to show your expertise and experience.

If you found these tips on writing a research hypothesis useful, head over to our blog on Statistical Hypothesis Testing to learn about the top researchers, papers, and institutions in this domain.

Frequently Asked Questions (FAQs)

1. what is the definition of hypothesis.

According to the Oxford dictionary, a hypothesis is defined as “An idea or explanation of something that is based on a few known facts, but that has not yet been proved to be true or correct”.

2. What is an example of hypothesis?

The hypothesis is a statement that proposes a relationship between two or more variables. An example: "If we increase the number of new users who join our platform by 25%, then we will see an increase in revenue."

3. What is an example of null hypothesis?

A null hypothesis is a statement that there is no relationship between two variables. The null hypothesis is written as H0. The null hypothesis states that there is no effect. For example, if you're studying whether or not a particular type of exercise increases strength, your null hypothesis will be "there is no difference in strength between people who exercise and people who don't."

4. What are the types of research?

• Fundamental research

• Applied research

• Qualitative research

• Quantitative research

• Mixed research

• Exploratory research

• Longitudinal research

• Cross-sectional research

• Field research

• Laboratory research

• Fixed research

• Flexible research

• Action research

• Policy research

• Classification research

• Comparative research

• Causal research

• Inductive research

• Deductive research

5. How to write a hypothesis?

• Your hypothesis should be able to predict the relationship and outcome.

• Avoid wordiness by keeping it simple and brief.

• Your hypothesis should contain observable and testable outcomes.

• Your hypothesis should be relevant to the research question.

6. What are the 2 types of hypothesis?

• Null hypotheses are used to test the claim that "there is no difference between two groups of data".

• Alternative hypotheses test the claim that "there is a difference between two data groups".

7. Difference between research question and research hypothesis?

A research question is a broad, open-ended question you will try to answer through your research. A hypothesis is a statement based on prior research or theory that you expect to be true due to your study. Example - Research question: What are the factors that influence the adoption of the new technology? Research hypothesis: There is a positive relationship between age, education and income level with the adoption of the new technology.

8. What is plural for hypothesis?

The plural of hypothesis is hypotheses. Here's an example of how it would be used in a statement, "Numerous well-considered hypotheses are presented in this part, and they are supported by tables and figures that are well-illustrated."

9. What is the red queen hypothesis?

The red queen hypothesis in evolutionary biology states that species must constantly evolve to avoid extinction because if they don't, they will be outcompeted by other species that are evolving. Leigh Van Valen first proposed it in 1973; since then, it has been tested and substantiated many times.

10. Who is known as the father of null hypothesis?

The father of the null hypothesis is Sir Ronald Fisher. He published a paper in 1925 that introduced the concept of null hypothesis testing, and he was also the first to use the term itself.

11. When to reject null hypothesis?

You need to find a significant difference between your two populations to reject the null hypothesis. You can determine that by running statistical tests such as an independent sample t-test or a dependent sample t-test. You should reject the null hypothesis if the p-value is less than 0.05.

formulation of hypothesis examples

You might also like

Consensus GPT vs. SciSpace GPT: Choose the Best GPT for Research

Consensus GPT vs. SciSpace GPT: Choose the Best GPT for Research

Sumalatha G

Literature Review and Theoretical Framework: Understanding the Differences

Nikhil Seethi

Types of Essays in Academic Writing - Quick Guide (2024)

  • Privacy Policy

Research Method

Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

What is a Hypothesis

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

  • Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
  • Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
  • Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
  • Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
  • Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
  • Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

  • Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
  • Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
  • Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
  • Education : “Implementing a new teaching method will result in higher student achievement scores.”
  • Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
  • Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
  • Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

  • In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
  • In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
  • I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

  • Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
  • Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
  • Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
  • Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
  • Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
  • Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
  • Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

  • Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
  • Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
  • Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
  • Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
  • Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
  • Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

  • Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
  • May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
  • May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
  • Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
  • Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
  • May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

About the author

' src=

Muhammad Hassan

Researcher, Academic Writer, Web developer

You may also like

Research Findings

Research Findings – Types Examples and Writing...

Informed Consent in Research

Informed Consent in Research – Types, Templates...

Research Methods

Research Methods – Types, Examples and Guide

Appendices

Appendices – Writing Guide, Types and Examples

Survey Instruments

Survey Instruments – List and Their Uses

Thesis Statement

Thesis Statement – Examples, Writing Guide

Encyclopedia Britannica

  • Games & Quizzes
  • History & Society
  • Science & Tech
  • Biographies
  • Animals & Nature
  • Geography & Travel
  • Arts & Culture
  • On This Day
  • One Good Fact
  • New Articles
  • Lifestyles & Social Issues
  • Philosophy & Religion
  • Politics, Law & Government
  • World History
  • Health & Medicine
  • Browse Biographies
  • Birds, Reptiles & Other Vertebrates
  • Bugs, Mollusks & Other Invertebrates
  • Environment
  • Fossils & Geologic Time
  • Entertainment & Pop Culture
  • Sports & Recreation
  • Visual Arts
  • Demystified
  • Image Galleries
  • Infographics
  • Top Questions
  • Britannica Kids
  • Saving Earth
  • Space Next 50
  • Student Center

experiments disproving spontaneous generation

  • When did science begin?
  • Where was science invented?

Blackboard inscribed with scientific formulas and calculations in physics and mathematics

scientific hypothesis

Our editors will review what you’ve submitted and determine whether to revise the article.

  • National Center for Biotechnology Information - PubMed Central - On the scope of scientific hypotheses
  • LiveScience - What is a scientific hypothesis?
  • The Royal Society - On the scope of scientific hypotheses

experiments disproving spontaneous generation

scientific hypothesis , an idea that proposes a tentative explanation about a phenomenon or a narrow set of phenomena observed in the natural world. The two primary features of a scientific hypothesis are falsifiability and testability, which are reflected in an “If…then” statement summarizing the idea and in the ability to be supported or refuted through observation and experimentation. The notion of the scientific hypothesis as both falsifiable and testable was advanced in the mid-20th century by Austrian-born British philosopher Karl Popper .

The formulation and testing of a hypothesis is part of the scientific method , the approach scientists use when attempting to understand and test ideas about natural phenomena. The generation of a hypothesis frequently is described as a creative process and is based on existing scientific knowledge, intuition , or experience. Therefore, although scientific hypotheses commonly are described as educated guesses, they actually are more informed than a guess. In addition, scientists generally strive to develop simple hypotheses, since these are easier to test relative to hypotheses that involve many different variables and potential outcomes. Such complex hypotheses may be developed as scientific models ( see scientific modeling ).

Depending on the results of scientific evaluation, a hypothesis typically is either rejected as false or accepted as true. However, because a hypothesis inherently is falsifiable, even hypotheses supported by scientific evidence and accepted as true are susceptible to rejection later, when new evidence has become available. In some instances, rather than rejecting a hypothesis because it has been falsified by new evidence, scientists simply adapt the existing idea to accommodate the new information. In this sense a hypothesis is never incorrect but only incomplete.

The investigation of scientific hypotheses is an important component in the development of scientific theory . Hence, hypotheses differ fundamentally from theories; whereas the former is a specific tentative explanation and serves as the main tool by which scientists gather data, the latter is a broad general explanation that incorporates data from many different scientific investigations undertaken to explore hypotheses.

Countless hypotheses have been developed and tested throughout the history of science . Several examples include the idea that living organisms develop from nonliving matter, which formed the basis of spontaneous generation , a hypothesis that ultimately was disproved (first in 1668, with the experiments of Italian physician Francesco Redi , and later in 1859, with the experiments of French chemist and microbiologist Louis Pasteur ); the concept proposed in the late 19th century that microorganisms cause certain diseases (now known as germ theory ); and the notion that oceanic crust forms along submarine mountain zones and spreads laterally away from them ( seafloor spreading hypothesis ).

Back Home

  • Science Notes Posts
  • Contact Science Notes
  • Todd Helmenstine Biography
  • Anne Helmenstine Biography
  • Free Printable Periodic Tables (PDF and PNG)
  • Periodic Table Wallpapers
  • Interactive Periodic Table
  • Periodic Table Posters
  • Science Experiments for Kids
  • How to Grow Crystals
  • Chemistry Projects
  • Fire and Flames Projects
  • Holiday Science
  • Chemistry Problems With Answers
  • Physics Problems
  • Unit Conversion Example Problems
  • Chemistry Worksheets
  • Biology Worksheets
  • Periodic Table Worksheets
  • Physical Science Worksheets
  • Science Lab Worksheets
  • My Amazon Books

Hypothesis Examples

Hypothesis Examples

A hypothesis is a prediction of the outcome of a test. It forms the basis for designing an experiment in the scientific method . A good hypothesis is testable, meaning it makes a prediction you can check with observation or experimentation. Here are different hypothesis examples.

Null Hypothesis Examples

The null hypothesis (H 0 ) is also known as the zero-difference or no-difference hypothesis. It predicts that changing one variable ( independent variable ) will have no effect on the variable being measured ( dependent variable ). Here are null hypothesis examples:

  • Plant growth is unaffected by temperature.
  • If you increase temperature, then solubility of salt will increase.
  • Incidence of skin cancer is unrelated to ultraviolet light exposure.
  • All brands of light bulb last equally long.
  • Cats have no preference for the color of cat food.
  • All daisies have the same number of petals.

Sometimes the null hypothesis shows there is a suspected correlation between two variables. For example, if you think plant growth is affected by temperature, you state the null hypothesis: “Plant growth is not affected by temperature.” Why do you do this, rather than say “If you change temperature, plant growth will be affected”? The answer is because it’s easier applying a statistical test that shows, with a high level of confidence, a null hypothesis is correct or incorrect.

Research Hypothesis Examples

A research hypothesis (H 1 ) is a type of hypothesis used to design an experiment. This type of hypothesis is often written as an if-then statement because it’s easy identifying the independent and dependent variables and seeing how one affects the other. If-then statements explore cause and effect. In other cases, the hypothesis shows a correlation between two variables. Here are some research hypothesis examples:

  • If you leave the lights on, then it takes longer for people to fall asleep.
  • If you refrigerate apples, they last longer before going bad.
  • If you keep the curtains closed, then you need less electricity to heat or cool the house (the electric bill is lower).
  • If you leave a bucket of water uncovered, then it evaporates more quickly.
  • Goldfish lose their color if they are not exposed to light.
  • Workers who take vacations are more productive than those who never take time off.

Is It Okay to Disprove a Hypothesis?

Yes! You may even choose to write your hypothesis in such a way that it can be disproved because it’s easier to prove a statement is wrong than to prove it is right. In other cases, if your prediction is incorrect, that doesn’t mean the science is bad. Revising a hypothesis is common. It demonstrates you learned something you did not know before you conducted the experiment.

Test yourself with a Scientific Method Quiz .

  • Mellenbergh, G.J. (2008). Chapter 8: Research designs: Testing of research hypotheses. In H.J. Adèr & G.J. Mellenbergh (eds.), Advising on Research Methods: A Consultant’s Companion . Huizen, The Netherlands: Johannes van Kessel Publishing.
  • Popper, Karl R. (1959). The Logic of Scientific Discovery . Hutchinson & Co. ISBN 3-1614-8410-X.
  • Schick, Theodore; Vaughn, Lewis (2002). How to think about weird things: critical thinking for a New Age . Boston: McGraw-Hill Higher Education. ISBN 0-7674-2048-9.
  • Tobi, Hilde; Kampen, Jarl K. (2018). “Research design: the methodology for interdisciplinary research framework”. Quality & Quantity . 52 (3): 1209–1225. doi: 10.1007/s11135-017-0513-8

Related Posts

Have a language expert improve your writing

Run a free plagiarism check in 10 minutes, generate accurate citations for free.

  • Knowledge Base

Hypothesis Testing | A Step-by-Step Guide with Easy Examples

Published on November 8, 2019 by Rebecca Bevans . Revised on June 22, 2023.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics . It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories.

There are 5 main steps in hypothesis testing:

  • State your research hypothesis as a null hypothesis and alternate hypothesis (H o ) and (H a  or H 1 ).
  • Collect data in a way designed to test the hypothesis.
  • Perform an appropriate statistical test .
  • Decide whether to reject or fail to reject your null hypothesis.
  • Present the findings in your results and discussion section.

Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps.

Table of contents

Step 1: state your null and alternate hypothesis, step 2: collect data, step 3: perform a statistical test, step 4: decide whether to reject or fail to reject your null hypothesis, step 5: present your findings, other interesting articles, frequently asked questions about hypothesis testing.

After developing your initial research hypothesis (the prediction that you want to investigate), it is important to restate it as a null (H o ) and alternate (H a ) hypothesis so that you can test it mathematically.

The alternate hypothesis is usually your initial hypothesis that predicts a relationship between variables. The null hypothesis is a prediction of no relationship between the variables you are interested in.

  • H 0 : Men are, on average, not taller than women. H a : Men are, on average, taller than women.

Here's why students love Scribbr's proofreading services

Discover proofreading & editing

For a statistical test to be valid , it is important to perform sampling and collect data in a way that is designed to test your hypothesis. If your data are not representative, then you cannot make statistical inferences about the population you are interested in.

There are a variety of statistical tests available, but they are all based on the comparison of within-group variance (how spread out the data is within a category) versus between-group variance (how different the categories are from one another).

If the between-group variance is large enough that there is little or no overlap between groups, then your statistical test will reflect that by showing a low p -value . This means it is unlikely that the differences between these groups came about by chance.

Alternatively, if there is high within-group variance and low between-group variance, then your statistical test will reflect that with a high p -value. This means it is likely that any difference you measure between groups is due to chance.

Your choice of statistical test will be based on the type of variables and the level of measurement of your collected data .

  • an estimate of the difference in average height between the two groups.
  • a p -value showing how likely you are to see this difference if the null hypothesis of no difference is true.

Based on the outcome of your statistical test, you will have to decide whether to reject or fail to reject your null hypothesis.

In most cases you will use the p -value generated by your statistical test to guide your decision. And in most cases, your predetermined level of significance for rejecting the null hypothesis will be 0.05 – that is, when there is a less than 5% chance that you would see these results if the null hypothesis were true.

In some cases, researchers choose a more conservative level of significance, such as 0.01 (1%). This minimizes the risk of incorrectly rejecting the null hypothesis ( Type I error ).

Prevent plagiarism. Run a free check.

The results of hypothesis testing will be presented in the results and discussion sections of your research paper , dissertation or thesis .

In the results section you should give a brief summary of the data and a summary of the results of your statistical test (for example, the estimated difference between group means and associated p -value). In the discussion , you can discuss whether your initial hypothesis was supported by your results or not.

In the formal language of hypothesis testing, we talk about rejecting or failing to reject the null hypothesis. You will probably be asked to do this in your statistics assignments.

However, when presenting research results in academic papers we rarely talk this way. Instead, we go back to our alternate hypothesis (in this case, the hypothesis that men are on average taller than women) and state whether the result of our test did or did not support the alternate hypothesis.

If your null hypothesis was rejected, this result is interpreted as “supported the alternate hypothesis.”

These are superficial differences; you can see that they mean the same thing.

You might notice that we don’t say that we reject or fail to reject the alternate hypothesis . This is because hypothesis testing is not designed to prove or disprove anything. It is only designed to test whether a pattern we measure could have arisen spuriously, or by chance.

If we reject the null hypothesis based on our research (i.e., we find that it is unlikely that the pattern arose by chance), then we can say our test lends support to our hypothesis . But if the pattern does not pass our decision rule, meaning that it could have arisen by chance, then we say the test is inconsistent with our hypothesis .

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

  • Normal distribution
  • Descriptive statistics
  • Measures of central tendency
  • Correlation coefficient

Methodology

  • Cluster sampling
  • Stratified sampling
  • Types of interviews
  • Cohort study
  • Thematic analysis

Research bias

  • Implicit bias
  • Cognitive bias
  • Survivorship bias
  • Availability heuristic
  • Nonresponse bias
  • Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Cite this Scribbr article

If you want to cite this source, you can copy and paste the citation or click the “Cite this Scribbr article” button to automatically add the citation to our free Citation Generator.

Bevans, R. (2023, June 22). Hypothesis Testing | A Step-by-Step Guide with Easy Examples. Scribbr. Retrieved June 24, 2024, from https://www.scribbr.com/statistics/hypothesis-testing/

Is this article helpful?

Rebecca Bevans

Rebecca Bevans

Other students also liked, choosing the right statistical test | types & examples, understanding p values | definition and examples, what is your plagiarism score.

  • Affiliate Program

Wordvice

  • UNITED STATES
  • 台灣 (TAIWAN)
  • TÜRKIYE (TURKEY)
  • Academic Editing Services
  • - Research Paper
  • - Journal Manuscript
  • - Dissertation
  • - College & University Assignments
  • Admissions Editing Services
  • - Application Essay
  • - Personal Statement
  • - Recommendation Letter
  • - Cover Letter
  • - CV/Resume
  • Business Editing Services
  • - Business Documents
  • - Report & Brochure
  • - Website & Blog
  • Writer Editing Services
  • - Script & Screenplay
  • Our Editors
  • Client Reviews
  • Editing & Proofreading Prices
  • Wordvice Points
  • Partner Discount
  • Plagiarism Checker
  • APA Citation Generator
  • MLA Citation Generator
  • Chicago Citation Generator
  • Vancouver Citation Generator
  • - APA Style
  • - MLA Style
  • - Chicago Style
  • - Vancouver Style
  • Writing & Editing Guide
  • Academic Resources
  • Admissions Resources

How to Write a Research Hypothesis: Good & Bad Examples

formulation of hypothesis examples

What is a research hypothesis?

A research hypothesis is an attempt at explaining a phenomenon or the relationships between phenomena/variables in the real world. Hypotheses are sometimes called “educated guesses”, but they are in fact (or let’s say they should be) based on previous observations, existing theories, scientific evidence, and logic. A research hypothesis is also not a prediction—rather, predictions are ( should be) based on clearly formulated hypotheses. For example, “We tested the hypothesis that KLF2 knockout mice would show deficiencies in heart development” is an assumption or prediction, not a hypothesis. 

The research hypothesis at the basis of this prediction is “the product of the KLF2 gene is involved in the development of the cardiovascular system in mice”—and this hypothesis is probably (hopefully) based on a clear observation, such as that mice with low levels of Kruppel-like factor 2 (which KLF2 codes for) seem to have heart problems. From this hypothesis, you can derive the idea that a mouse in which this particular gene does not function cannot develop a normal cardiovascular system, and then make the prediction that we started with. 

What is the difference between a hypothesis and a prediction?

You might think that these are very subtle differences, and you will certainly come across many publications that do not contain an actual hypothesis or do not make these distinctions correctly. But considering that the formulation and testing of hypotheses is an integral part of the scientific method, it is good to be aware of the concepts underlying this approach. The two hallmarks of a scientific hypothesis are falsifiability (an evaluation standard that was introduced by the philosopher of science Karl Popper in 1934) and testability —if you cannot use experiments or data to decide whether an idea is true or false, then it is not a hypothesis (or at least a very bad one).

So, in a nutshell, you (1) look at existing evidence/theories, (2) come up with a hypothesis, (3) make a prediction that allows you to (4) design an experiment or data analysis to test it, and (5) come to a conclusion. Of course, not all studies have hypotheses (there is also exploratory or hypothesis-generating research), and you do not necessarily have to state your hypothesis as such in your paper. 

But for the sake of understanding the principles of the scientific method, let’s first take a closer look at the different types of hypotheses that research articles refer to and then give you a step-by-step guide for how to formulate a strong hypothesis for your own paper.

Types of Research Hypotheses

Hypotheses can be simple , which means they describe the relationship between one single independent variable (the one you observe variations in or plan to manipulate) and one single dependent variable (the one you expect to be affected by the variations/manipulation). If there are more variables on either side, you are dealing with a complex hypothesis. You can also distinguish hypotheses according to the kind of relationship between the variables you are interested in (e.g., causal or associative ). But apart from these variations, we are usually interested in what is called the “alternative hypothesis” and, in contrast to that, the “null hypothesis”. If you think these two should be listed the other way round, then you are right, logically speaking—the alternative should surely come second. However, since this is the hypothesis we (as researchers) are usually interested in, let’s start from there.

Alternative Hypothesis

If you predict a relationship between two variables in your study, then the research hypothesis that you formulate to describe that relationship is your alternative hypothesis (usually H1 in statistical terms). The goal of your hypothesis testing is thus to demonstrate that there is sufficient evidence that supports the alternative hypothesis, rather than evidence for the possibility that there is no such relationship. The alternative hypothesis is usually the research hypothesis of a study and is based on the literature, previous observations, and widely known theories. 

Null Hypothesis

The hypothesis that describes the other possible outcome, that is, that your variables are not related, is the null hypothesis ( H0 ). Based on your findings, you choose between the two hypotheses—usually that means that if your prediction was correct, you reject the null hypothesis and accept the alternative. Make sure, however, that you are not getting lost at this step of the thinking process: If your prediction is that there will be no difference or change, then you are trying to find support for the null hypothesis and reject H1. 

Directional Hypothesis

While the null hypothesis is obviously “static”, the alternative hypothesis can specify a direction for the observed relationship between variables—for example, that mice with higher expression levels of a certain protein are more active than those with lower levels. This is then called a one-tailed hypothesis. 

Another example for a directional one-tailed alternative hypothesis would be that 

H1: Attending private classes before important exams has a positive effect on performance. 

Your null hypothesis would then be that

H0: Attending private classes before important exams has no/a negative effect on performance.

Nondirectional Hypothesis

A nondirectional hypothesis does not specify the direction of the potentially observed effect, only that there is a relationship between the studied variables—this is called a two-tailed hypothesis. For instance, if you are studying a new drug that has shown some effects on pathways involved in a certain condition (e.g., anxiety) in vitro in the lab, but you can’t say for sure whether it will have the same effects in an animal model or maybe induce other/side effects that you can’t predict and potentially increase anxiety levels instead, you could state the two hypotheses like this:

H1: The only lab-tested drug (somehow) affects anxiety levels in an anxiety mouse model.

You then test this nondirectional alternative hypothesis against the null hypothesis:

H0: The only lab-tested drug has no effect on anxiety levels in an anxiety mouse model.

hypothesis in a research paper

How to Write a Hypothesis for a Research Paper

Now that we understand the important distinctions between different kinds of research hypotheses, let’s look at a simple process of how to write a hypothesis.

Writing a Hypothesis Step:1

Ask a question, based on earlier research. Research always starts with a question, but one that takes into account what is already known about a topic or phenomenon. For example, if you are interested in whether people who have pets are happier than those who don’t, do a literature search and find out what has already been demonstrated. You will probably realize that yes, there is quite a bit of research that shows a relationship between happiness and owning a pet—and even studies that show that owning a dog is more beneficial than owning a cat ! Let’s say you are so intrigued by this finding that you wonder: 

What is it that makes dog owners even happier than cat owners? 

Let’s move on to Step 2 and find an answer to that question.

Writing a Hypothesis Step 2:

Formulate a strong hypothesis by answering your own question. Again, you don’t want to make things up, take unicorns into account, or repeat/ignore what has already been done. Looking at the dog-vs-cat papers your literature search returned, you see that most studies are based on self-report questionnaires on personality traits, mental health, and life satisfaction. What you don’t find is any data on actual (mental or physical) health measures, and no experiments. You therefore decide to make a bold claim come up with the carefully thought-through hypothesis that it’s maybe the lifestyle of the dog owners, which includes walking their dog several times per day, engaging in fun and healthy activities such as agility competitions, and taking them on trips, that gives them that extra boost in happiness. You could therefore answer your question in the following way:

Dog owners are happier than cat owners because of the dog-related activities they engage in.

Now you have to verify that your hypothesis fulfills the two requirements we introduced at the beginning of this resource article: falsifiability and testability . If it can’t be wrong and can’t be tested, it’s not a hypothesis. We are lucky, however, because yes, we can test whether owning a dog but not engaging in any of those activities leads to lower levels of happiness or well-being than owning a dog and playing and running around with them or taking them on trips.  

Writing a Hypothesis Step 3:

Make your predictions and define your variables. We have verified that we can test our hypothesis, but now we have to define all the relevant variables, design our experiment or data analysis, and make precise predictions. You could, for example, decide to study dog owners (not surprising at this point), let them fill in questionnaires about their lifestyle as well as their life satisfaction (as other studies did), and then compare two groups of active and inactive dog owners. Alternatively, if you want to go beyond the data that earlier studies produced and analyzed and directly manipulate the activity level of your dog owners to study the effect of that manipulation, you could invite them to your lab, select groups of participants with similar lifestyles, make them change their lifestyle (e.g., couch potato dog owners start agility classes, very active ones have to refrain from any fun activities for a certain period of time) and assess their happiness levels before and after the intervention. In both cases, your independent variable would be “ level of engagement in fun activities with dog” and your dependent variable would be happiness or well-being . 

Examples of a Good and Bad Hypothesis

Let’s look at a few examples of good and bad hypotheses to get you started.

Good Hypothesis Examples

Working from home improves job satisfaction.Employees who are allowed to work from home are less likely to quit within 2 years than those who need to come to the office.
Sleep deprivation affects cognition.Students who sleep <5 hours/night don’t perform as well on exams as those who sleep >7 hours/night. 
Animals adapt to their environment.Birds of the same species living on different islands have differently shaped beaks depending on the available food source.
Social media use causes anxiety.Do teenagers who refrain from using social media for 4 weeks show improvements in anxiety symptoms?

Bad Hypothesis Examples

Garlic repels vampires.Participants who eat garlic daily will not be harmed by vampires.Nobody gets harmed by vampires— .
Chocolate is better than vanilla.           No clearly defined variables— .

Tips for Writing a Research Hypothesis

If you understood the distinction between a hypothesis and a prediction we made at the beginning of this article, then you will have no problem formulating your hypotheses and predictions correctly. To refresh your memory: We have to (1) look at existing evidence, (2) come up with a hypothesis, (3) make a prediction, and (4) design an experiment. For example, you could summarize your dog/happiness study like this:

(1) While research suggests that dog owners are happier than cat owners, there are no reports on what factors drive this difference. (2) We hypothesized that it is the fun activities that many dog owners (but very few cat owners) engage in with their pets that increases their happiness levels. (3) We thus predicted that preventing very active dog owners from engaging in such activities for some time and making very inactive dog owners take up such activities would lead to an increase and decrease in their overall self-ratings of happiness, respectively. (4) To test this, we invited dog owners into our lab, assessed their mental and emotional well-being through questionnaires, and then assigned them to an “active” and an “inactive” group, depending on… 

Note that you use “we hypothesize” only for your hypothesis, not for your experimental prediction, and “would” or “if – then” only for your prediction, not your hypothesis. A hypothesis that states that something “would” affect something else sounds as if you don’t have enough confidence to make a clear statement—in which case you can’t expect your readers to believe in your research either. Write in the present tense, don’t use modal verbs that express varying degrees of certainty (such as may, might, or could ), and remember that you are not drawing a conclusion while trying not to exaggerate but making a clear statement that you then, in a way, try to disprove . And if that happens, that is not something to fear but an important part of the scientific process.

Similarly, don’t use “we hypothesize” when you explain the implications of your research or make predictions in the conclusion section of your manuscript, since these are clearly not hypotheses in the true sense of the word. As we said earlier, you will find that many authors of academic articles do not seem to care too much about these rather subtle distinctions, but thinking very clearly about your own research will not only help you write better but also ensure that even that infamous Reviewer 2 will find fewer reasons to nitpick about your manuscript. 

Perfect Your Manuscript With Professional Editing

Now that you know how to write a strong research hypothesis for your research paper, you might be interested in our free AI Proofreader , Wordvice AI, which finds and fixes errors in grammar, punctuation, and word choice in academic texts. Or if you are interested in human proofreading , check out our English editing services , including research paper editing and manuscript editing .

On the Wordvice academic resources website , you can also find many more articles and other resources that can help you with writing the other parts of your research paper , with making a research paper outline before you put everything together, or with writing an effective cover letter once you are ready to submit.

  • Thesis Action Plan New
  • Academic Project Planner

Literature Navigator

Thesis dialogue blueprint, writing wizard's template, research proposal compass.

  • Why students love us
  • Why professors love us
  • Rebels Blog (Free)
  • Why we are different
  • All Products
  • Coming Soon

Exploring Research Question and Hypothesis Examples: A Comprehensive Guide

Exploring Research Question and Hypothesis Examples: A Comprehensive Guide

This comprehensive guide explores the intricacies of formulating research questions and hypotheses across various academic disciplines. By delving into examples and methodological approaches, the article aims to provide scholars and researchers with the tools necessary to develop robust and effective research frameworks. Understanding and crafting well-formed research questions and hypotheses are pivotal in conducting meaningful research that can significantly contribute to knowledge within a field.

Key Takeaways

  • Understand the fundamental differences and connections between research questions and hypotheses.
  • Learn how to craft effective and precise research questions that guide the research process.
  • Explore various types of hypotheses and methods for testing and refining them.
  • Examine practical examples of research questions and hypotheses across multiple disciplines.
  • Gain insights into the impact of well-constructed research questions and hypotheses on research outcomes, academic publishing, and grant applications.

Understanding the Fundamentals of Research Questions and Hypotheses

Defining research questions.

Research questions are the backbone of any scholarly inquiry, guiding you through the exploration of your chosen topic. They help you focus your study and determine the direction of your research. A well-crafted research question should be clear, focused, and answerable within the constraints of your study.

Characteristics of a Strong Hypothesis

A strong hypothesis provides a specific, testable prediction about the expected outcomes of your research. It is not merely a guess but is grounded in existing literature and theory. To develop a robust hypothesis, consider the variables involved and ensure that it is feasible to test them within your study's design.

Interrelation Between Research Questions and Hypotheses

Understanding the interrelation between research questions and hypotheses is crucial for structuring your research effectively. Your hypothesis should directly address the gap in the literature highlighted by your research question, providing a clear pathway for investigation. This alignment ensures that your study can contribute valuable insights to your field.

Crafting Effective Research Questions

Identifying the purpose.

To craft an effective research question , you must first identify the purpose of your study. This involves understanding what you aim to discover or elucidate through your research. Ask yourself what the core of your inquiry is and what outcomes you hope to achieve. This clarity will guide your entire research process, ensuring that your question is not only relevant but also deeply rooted in your specific academic or practical goals.

Scope and Limitations

It's crucial to define the scope and limitations of your research early on. This helps in setting realistic boundaries and expectations for your study. Consider factors such as time, resources, and the breadth of the subject area. Narrowing down your focus to a manageable scope can prevent the common pitfall of an overly broad or vague question, which can dilute the impact of your findings.

Formulating Questions that Drive Inquiry

The final step in crafting your research question is formulating it in a way that drives inquiry. This means your question should be clear, concise, and structured to prompt detailed investigation and critical analysis. It should challenge existing knowledge and push the boundaries of what is already known. Utilizing strategies like the Thesis Dialogue Blueprint or the Research Proposal Compass can be instrumental in refining your question to ensure it is both innovative and feasible.

Developing Hypotheses in Research

From research questions to hypotheses.

When you transition from research questions to hypotheses, you are essentially moving from what you want to know to what you predict will happen. This shift involves formulating a specific, testable prediction that directly stems from your initial question. Ensure your hypothesis is directly linked to and derived from your research question to maintain a coherent research strategy.

Types of Hypotheses

There are several types of hypotheses you might encounter, including simple, complex, directional, nondirectional, associative, causal, null, and alternative. Each type serves a different purpose and is chosen based on the specifics of the research question and the nature of the study. For instance, a null hypothesis might be used to test the effectiveness of a new teaching method compared to the standard.

Testing and Refining Hypotheses

Testing your hypothesis is a critical step in the research process. This phase involves collecting data, conducting experiments, or utilizing other research methods to determine the validity of your hypothesis. After testing, you may find that your hypothesis needs refining or even reformation based on the outcomes. This iterative process is essential for narrowing down the most accurate explanation or prediction for your research question.

Examples of Research Questions in Various Disciplines

Humanities and social sciences.

In the realm of Humanities and Social Sciences, research questions often explore cultural, social, historical, or philosophical aspects. How does gender representation in 20th-century American literature reflect broader social changes? This question not only seeks to uncover specific literary trends but also ties them to societal shifts, offering a rich field for analysis.

Natural Sciences

Research questions in the Natural Sciences are typically aimed at understanding natural phenomena or solving specific scientific problems. A common question might be, What are the effects of plastic pollutants on marine biodiversity? This inquiry highlights the environmental concerns and seeks empirical data to understand the impact.

Applied Sciences

In Applied Sciences, the focus is often on improving technology or engineering solutions. A pertinent question could be, How can renewable energy sources be integrated into existing power grids? This question addresses the practical challenges and potential innovations in energy systems, crucial for advancing sustainable technologies.

Analyzing Hypothesis Examples Across Fields

Case studies in psychology.

In psychology, hypotheses often explore the causal relationships between cognitive functions and behaviors. Consider how a hypothesis might predict the impact of stress on memory recall . By examining various case studies, you can see how hypotheses are specifically tailored to address intricate psychological phenomena.

Experimental Research in Biology

Biology experiments frequently test hypotheses about physiological processes or genetic information. For instance, a hypothesis might propose that a specific gene influences plant growth rates. Through rigorous testing, these hypotheses contribute significantly to our understanding of biological systems.

Field Studies in Environmental Science

Field studies in environmental science provide a rich ground for testing hypotheses related to ecosystem dynamics and conservation strategies. A common hypothesis might explore the effects of human activity on biodiversity. These studies often involve complex data collection and analysis, highlighting the interrelation between empirical evidence and theoretical predictions.

Methodological Approaches to Formulating Hypotheses

Quantitative vs. qualitative research.

When you embark on hypothesis formulation, understanding the distinction between quantitative and qualitative research methodologies is crucial. Quantitative research focuses on numerical data and statistical analysis, ideal for hypotheses that require measurable evidence. In contrast, qualitative research delves into thematic and descriptive data, providing depth and context to hypotheses that explore behaviors, perceptions, and experiences.

The Role of Theoretical Frameworks

Theoretical frameworks serve as the backbone for developing robust hypotheses. They provide a structured way to align your hypothesis with existing knowledge. By integrating theories and models relevant to your study, you ensure that your hypothesis has a solid foundation and aligns with established academic thought.

Utilizing Existing Literature to Form Hypotheses

A thorough review of existing literature is indispensable for crafting a well-informed hypothesis. This process not only highlights gaps in current research but also allows you to build on the work of others. By synthesizing findings from previous studies, you can formulate hypotheses that are both innovative and grounded in academic precedent.

Evaluating the Impact of Well-Formed Research Questions and Hypotheses

On research outcomes.

Understanding the impact of well-formed research questions and hypotheses on research outcomes is crucial. Well-crafted questions and hypotheses serve as a framework that guides the entire research process , ensuring that the study remains focused and relevant. They help in defining the scope of the study and in identifying the variables that need to be measured, thus directly influencing the validity and reliability of the research findings.

In Academic Publishing

The role of well-defined research questions and hypotheses extends beyond the research process into the realm of academic publishing. A clear hypothesis provides a strong foundation for the research paper, enhancing its chances of acceptance in prestigious journals. The clarity and direction afforded by a solid hypothesis make the research more appealing to a scholarly audience, potentially increasing citation rates and academic recognition.

In Grant Applications

When applying for research grants, the clarity of your research questions and hypotheses can significantly impact the decision-making process of funding bodies. A well-articulated hypothesis demonstrates a clear vision and a structured approach to addressing a specific issue, which can be crucial in securing funding. Grant reviewers often look for proposals that promise substantial contributions to the field, and a strong hypothesis can be a key factor in showcasing the potential impact of your research.

In our latest article, 'Evaluating the Impact of Well-Formed Research Questions and Hypotheses,' we delve into the crucial role that precise questions and hypotheses play in academic research. Understanding this can significantly enhance your thesis writing process. For a deeper exploration and practical tools to apply these concepts, visit our website and discover how our Thesis Action Plan can transform your academic journey. Don't miss out on our special offers tailored just for you!

In this comprehensive guide, we have explored various examples of research questions and hypotheses, shedding light on their significance and application in academic research. Understanding the distinction between a research question and a hypothesis, as well as knowing how to effectively formulate them, is crucial for conducting methodical and impactful studies. By examining different scenarios and examples, this guide aims to equip researchers with the knowledge to craft well-defined research questions and hypotheses that can drive meaningful investigations and contribute to the broader field of knowledge. As we continue to delve into the intricacies of research design, it is our hope that this guide serves as a valuable resource for both novice and experienced researchers in their scholarly endeavors.

Frequently Asked Questions

What is a research question.

A research question is a clearly defined query that guides a scientific or academic study. It sets the scope and focus of the research by asking about a specific phenomenon or issue.

How does a hypothesis differ from a research question?

A hypothesis is a specific, testable prediction about what will happen in a study based on prior knowledge or theory, while a research question is an open query that guides the direction of the investigation.

What are the characteristics of a strong hypothesis?

A strong hypothesis is clear, testable, based on existing knowledge, and it states an expected relationship between variables.

How can research questions and hypotheses interrelate?

Research questions define the scope of inquiry, while hypotheses provide a specific prediction about the expected outcomes that can be tested through research methods.

What should be considered when formulating a research question?

When formulating a research question, consider clarity, focus, relevance, and the feasibility of answering the question through available research methods.

Why is it important to have a well-formed hypothesis?

A well-formed hypothesis directs the research process, allows for clear testing of assumptions, and helps in drawing meaningful conclusions that can contribute to the body of knowledge.

10 Effective Strategies for Research Question Help

What Is the 3 3 3 Rule for Anxiety? A Comprehensive Guide

How to deal with thesis burnout: tips and strategies, what are the symptoms of thesis burnout identifying the signs, how to get over thesis anxiety: effective techniques, why is my thesis so hard understanding the challenges.

Avoiding Procrastination Pitfalls: Bachelor Thesis Progress and Weekend Celebrations

Avoiding Procrastination Pitfalls: Bachelor Thesis Progress and Weekend Celebrations

How Do You Write a Hypothesis for a Research Paper? Step-by-Step Guide

How Do You Write a Hypothesis for a Research Paper? Step-by-Step Guide

How to Write a Thesis Fast: Tips and Strategies for Success

How to Write a Thesis Fast: Tips and Strategies for Success

The Note-Taking Debate: Pros and Cons of Digital and Analog Methods

The Note-Taking Debate: Pros and Cons of Digital and Analog Methods

Maximize Your Academic Excellence with These 9 Evening Habits for Quality Sleep

Maximize Your Academic Excellence with These 9 Evening Habits for Quality Sleep

Comprehensive Thesis Guide

Thesis Action Plan

Research Proposal Compass

  • Rebels Blog
  • Blog Articles
  • Terms and Conditions
  • Payment and Shipping Terms
  • Privacy Policy
  • Return Policy

© 2024 Research Rebels, All rights reserved.

Your cart is currently empty.

Research Hypothesis In Psychology: Types, & Examples

Saul Mcleod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul Mcleod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

Learn about our Editorial Process

Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

On This Page:

A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

  • A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
  • It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
  • A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
  • Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
  • For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
  • Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.
Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

  • Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

hypothesis

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.
  • Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
  • However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

  • Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
  • Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
  • Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
  • Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
  • Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

  • The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
  • The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

  • Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
  • Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
  • Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
  • Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
  • Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
  • Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
  • Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
  • Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

Print Friendly, PDF & Email

Related Articles

Mixed Methods Research

Research Methodology

Mixed Methods Research

Conversation Analysis

Conversation Analysis

Discourse Analysis

Discourse Analysis

Phenomenology In Qualitative Research

Phenomenology In Qualitative Research

Ethnography In Qualitative Research

Ethnography In Qualitative Research

Narrative Analysis In Qualitative Research

Narrative Analysis In Qualitative Research

Find Study Materials for

  • Explanations
  • Business Studies
  • Combined Science
  • Engineering
  • English Literature
  • Environmental Science
  • Human Geography
  • Macroeconomics
  • Microeconomics
  • Social Studies
  • Browse all subjects
  • Read our Magazine

Create Study Materials

  • Flashcards Create and find the best flashcards.
  • Notes Create notes faster than ever before.
  • Study Sets Everything you need for your studies in one place.
  • Study Plans Stop procrastinating with our smart planner features.
  • Formulation of Hypothesis

Children who spend more time playing outside are more likely to be imaginative. What do you think this statement is an example of in terms of scientific research ? If you guessed a hypothesis, then you'd be correct. The formulation of hypotheses is a fundamental step in psychology research.

Formulation of Hypothesis

Create learning materials about Formulation of Hypothesis with our free learning app!

  • Instand access to millions of learning materials
  • Flashcards, notes, mock-exams and more
  • Everything you need to ace your exams
  • Approaches in Psychology
  • Basic Psychology
  • Biological Bases of Behavior
  • Biopsychology
  • Careers in Psychology
  • Clinical Psychology
  • Bartlett War of the Ghosts
  • Brain Development
  • Bruner and Minturn Study of Perceptual Set
  • Case Studies Psychology
  • Computation
  • Conservation of Number Piaget
  • Constructive Processes in Memory
  • Correlation
  • Data handling
  • Depth Cues Psychology
  • Designing Research
  • Developmental Research
  • Dweck's Theory of Mindset
  • Ethical considerations in research
  • Experimental Method
  • Factors Affecting Perception
  • Factors Affecting the Accuracy of Memory
  • Gibson's Theory of Direct Perception
  • Gregory's Constructivist Theory of Perception
  • Gunderson et al 2013 study
  • Hughes Policeman Doll Study
  • Issues and Debates in Developmental Psychology
  • Language and Perception
  • McGarrigle and Donaldson Naughty Teddy
  • Memory Processes
  • Memory recall
  • Nature and Nurture in Development
  • Normal Distribution Psychology
  • Perception Research
  • Perceptual Set
  • Piagets Theory in Education
  • Planning and Conducting Research
  • Population Samples
  • Primary and Secondary Data
  • Quantitative Data
  • Quantitative and Qualitative Data
  • Quantitative and Qualitative Methods
  • Research Procedures
  • Serial Position Effect
  • Short-term Retention
  • Structures of Memory
  • Tables, Charts and Graphs
  • The Effects of Learning on Development
  • The Gilchrist And Nesberg Study Of Motivation
  • Three Mountains Task
  • Types of Variable
  • Types of bias and how to control
  • Visual Cues and Constancies
  • Visual illusions
  • Willingham's Learning Theory
  • Cognition and Development
  • Cognitive Psychology
  • Data Handling and Analysis
  • Developmental Psychology
  • Eating Behaviour
  • Emotion and Motivation
  • Famous Psychologists
  • Forensic Psychology
  • Health Psychology
  • Individual Differences Psychology
  • Issues and Debates in Psychology
  • Personality in Psychology
  • Psychological Treatment
  • Relationships
  • Research Methods in Psychology
  • Schizophrenia
  • Scientific Foundations of Psychology
  • Scientific Investigation
  • Sensation and Perception
  • Social Context of Behaviour
  • Social Psychology
  • First, we will discuss the importance of hypotheses in research.
  • We will then cover formulating hypotheses in research, including the steps in the formulation of hypotheses in research methodology.
  • We will provide examples of hypotheses in research throughout the explanation.
  • Finally, we will delve into the different types of hypotheses in research.

What is a Hypothesis?

The current community of psychologists believe that the best approach to understanding behaviour is to conduct scientific research . To be classed as scientific research , it must be observable, valid, reliable and follow a standardised procedure.

One of the important steps in scientific research is to formulate a hypothesis before starting the study procedure.

The hypothesis is a predictive, testable statement predicting the outcome and the results the researcher expects to find.

The hypothesis provides a summary of what direction, if any, is taken to investigate a theory.

In scientific research, there is a criterion that hypotheses need to be met to be regarded as acceptable.

If a hypothesis is disregarded, the research may be rejected by the community of psychology researchers.

Importance of Hypothesis in Research

The purpose of including hypotheses in psychology research is:

  • To provide a summary of the research, how it will be investigated, and what is expected to be found.
  • To provide an answer to the research question.

When carrying out research, researchers first investigate the research area they are interested in. From this, researchers are required to identify a gap in the literature.

Filling the gap essentially means finding what previous work has not been explained yet, investigated to a sufficient degree, or simply expanding or further investigating a theory if doubt exists.

The researcher then forms a research question that the researcher will attempt to answer in their study.

Remember, the hypothesis is a predictive statement of what is expected to happen when testing the research question.

The hypothesis can be used for later data analysis. This includes inferential tests such as hypothesis testing and identifying if statistical findings are significant.

Formulation of testable hypotheses, four people with question marks above their heads, StudySmarter

Steps in the Formulation of Hypothesis in Research Methodology

Researchers must follow certain steps to formulate testable hypotheses when conducting research.

Overall, the researcher has to consider the direction of the research, i.e. will it be looking for a difference caused by independent variables ? Or will it be more concerned with the correlation between variables?

All researchers will likely complete the following.

  • Investigating background research in the area of interest.
  • Formulating or investigating a theory.
  • Identify how the theory will be tested and what the researcher expects to find based on relevant, previously published scientific works.

The above steps are used to formulate testable hypotheses.

The Formulation of Testable Hypotheses

The hypothesis is important in research as it indicates what and how a variable will be investigated.

The hypothesis essentially summarises what and how something will be investigated. This is important as it ensures that the researcher has carefully planned how the research will be done, as the researchers have to follow a set procedure to conduct research.

This is known as the scientific method.

Formulating Hypotheses in Research

When formulating hypotheses, things that researchers should consider are:

Hypothesis RequirementDescription
It should be written as predictive statements regarding the relationship between the IV and DV.The researcher should be able to predict what they expect to find from the study results. The researcher could state that they expect to see a difference. Occasionally, researchers may theorise what changes are expected to be observed (two-tailed alternative hypothesis).
It should be formulated based on background research.Hypotheses should not be based on guesswork. Instead, researchers should use previously published research to predict the study's expected outcome.
Identify the IV. IV is what the experimenter manipulates to see if it affects the DV.
Identify the DV.DV is the variable being measured after the IV has been manipulated or after it changes during the experiment.
The should be operationalised. The researchers must define how each variable (IV and DV) will be measured. For example, may be measured using a performance test, such as the Mini-Mental Status Examination. When a hypothesis is operationalised, it is testable.
The hypotheses need to be falsifiable.Other researchers need to be able to replicate the research using the same variables to see whether they can verify the results. The hypothesis needs to be written in a way that is falsifiable, meaning it can be tested using the scientific method to see if it is true.An example of a non-falsifiable hypothesis is "leprechauns always find the pot of gold at the end of the rainbow."
The hypotheses should be clear. Hypotheses are usually only a sentence long and should only include the details summarised above. A good hypothesis should not include irrelevant information.

Types of Hypotheses in Research

Researchers can propose different types of hypotheses when carrying out research.

The following research scenario will be discussed to show examples of each type of hypothesis that the researchers could use. "A research team was investigating whether memory performance is affected by depression ."

The identified independent variable is the severity of depression scores, and the dependent variable is the scores from a memory performance task.

The null hypothesis predicts that the results will show no or little effect. The null hypothesis is a predictive statement that researchers use when it is thought that the IV will not influence the DV.

In this case, the null hypothesis would be there will be no difference in memory scores on the MMSE test of those who are diagnosed with depression and those who are not.

An alternative hypothesis is a predictive statement used when it is thought that the IV will influence the DV. The alternative hypothesis is also called a non-directional, two-tailed hypothesis, as it predicts the results can go either way, e.g. increase or decrease.

The example in this scenario is there will be an observed difference in scores from a memory performance task between people with high- or low-depressive scores.

The directional alternative hypothesis states how the IV will influence the DV, identifying a specific direction, such as if there will be an increase or decrease in the observed results.

The example in this scenario is people with low depressive scores will perform better in the memory performance task than people who score higher in depressive symptoms.

Example Hypothesis in Research

To summarise, let's look at an example of a straightforward hypothesis that indicates the relationship between two variables: the independent and the dependent.

If you stay up late, you will feel tired the following day; the more caffeine you drink, the harder you find it to fall asleep, or the more sunlight plants get, the taller they will grow.

Formulation of Hypothesis - Key Takeaways

  • The current community of psychologists believe that the best approach to understanding behaviour is to conduct scientific research. One of the important steps in scientific research is to create a hypothesis.
  • The hypothesis is a predictive, testable statement concerning the outcome/results that the researcher expects to find.
  • Hypotheses are needed in research to provide a summary of what the research is, how to investigate a theory and what is expected to be found, and to provide an answer to the research question so that the hypothesis can be used for later data analysis.
  • There are requirements for the formulation of testable hypotheses. The hypotheses should identify and operationalise the IV and DV. In addition, they should describe the nature of the relationship between the IV and DV.
  • There are different types of hypotheses: Null hypothesis, Alternative hypothesis (this is also known as the non-directional, two-tailed hypothesis), and Directional hypothesis (this is also known as the one-tailed hypothesis).

Flashcards in Formulation of Hypothesis 18

What type of hypothesis matches the following definition. A predictive statement that researchers use when it is thought that the IV will not influence the DV.

Null hypothesis 

What type of hypothesis matches the following definition. A hypothesis that states that the IV will influence the DV. But, the hypothesis does not state how the IV will influence the DV. 

Alternative hypothesis 

What type of hypothesis matches the following definition. A hypothesis that states that the IV will influence the DV, and states how it will influence the DV. 

Directional, alternative hypothesis 

Which type of hypothesis is also known as a two-tailed hypothesis? 

What type of hypothesis is the following example. There will be no observed difference in scores from a memory performance task between people with high- or low-depressive scores.

What type of hypothesis is the following example. There will be an observed difference in scores from a memory performance task between people with high- or low-depressive scores.

Formulation of Hypothesis

Learn with 18 Formulation of Hypothesis flashcards in the free StudySmarter app

We have 14,000 flashcards about Dynamic Landscapes.

Already have an account? Log in

Frequently Asked Questions about Formulation of Hypothesis

What are the 3 types of hypotheses?

The three types of hypotheses are:

  • Null hypothesis 
  • Alternative hypothesis 
  • Directional/non-directional hypothesis 

What is an example of a hypothesis in psychology?

An example of a null hypothesis in psychology is, there will be no observed difference in scores from a memory performance task between people with high- or low-depressive scores.

What are the steps in formulating a hypothesis?

All researchers will likely complete the following

  • Investigating background research in the area of interest 
  • Formulating or investigating a theory 
  • Identify how the theory will be tested and what the researcher expects to find based on relevant, previously published scientific works 

What is formulation of hypothesis in research? 

The formulation of a hypothesis in research is when the researcher formulates a predictive statement of what is expected to happen when testing the research question based on background research.

How to formulate  null and alternative hypothesis?

When formulating a null hypothesis the researcher would state a prediction that they expect to see no difference in the dependent variable when the independent variable changes or is manipulated. Whereas, when using an alternative hypothesis then it would be predicted that there will be a change in the dependent variable. The researcher can state in which direction they expect the results to go. 

Test your knowledge with multiple choice flashcards

Formulation of Hypothesis

Join the StudySmarter App and learn efficiently with millions of flashcards and more!

Keep learning, you are doing great.

Discover learning materials with the free StudySmarter app

1

About StudySmarter

StudySmarter is a globally recognized educational technology company, offering a holistic learning platform designed for students of all ages and educational levels. Our platform provides learning support for a wide range of subjects, including STEM, Social Sciences, and Languages and also helps students to successfully master various tests and exams worldwide, such as GCSE, A Level, SAT, ACT, Abitur, and more. We offer an extensive library of learning materials, including interactive flashcards, comprehensive textbook solutions, and detailed explanations. The cutting-edge technology and tools we provide help students create their own learning materials. StudySmarter’s content is not only expert-verified but also regularly updated to ensure accuracy and relevance.

Formulation of Hypothesis

StudySmarter Editorial Team

Team Psychology Teachers

  • 8 minutes reading time
  • Checked by StudySmarter Editorial Team

Study anywhere. Anytime.Across all devices.

Create a free account to save this explanation..

Save explanations to your personalised space and access them anytime, anywhere!

By signing up, you agree to the Terms and Conditions and the Privacy Policy of StudySmarter.

Sign up to highlight and take notes. It’s 100% free.

Join over 22 million students in learning with our StudySmarter App

The first learning app that truly has everything you need to ace your exams in one place

  • Flashcards & Quizzes
  • AI Study Assistant
  • Study Planner
  • Smart Note-Taking

Join over 22 million students in learning with our StudySmarter App

Get unlimited access with a free StudySmarter account.

  • Instant access to millions of learning materials.
  • Flashcards, notes, mock-exams, AI tools and more.
  • Everything you need to ace your exams.

Second Popup Banner

Definition of a Hypothesis

What it is and how it's used in sociology

  • Key Concepts
  • Major Sociologists
  • News & Issues
  • Research, Samples, and Statistics
  • Recommended Reading
  • Archaeology

A hypothesis is a prediction of what will be found at the outcome of a research project and is typically focused on the relationship between two different variables studied in the research. It is usually based on both theoretical expectations about how things work and already existing scientific evidence.

Within social science, a hypothesis can take two forms. It can predict that there is no relationship between two variables, in which case it is a null hypothesis . Or, it can predict the existence of a relationship between variables, which is known as an alternative hypothesis.

In either case, the variable that is thought to either affect or not affect the outcome is known as the independent variable, and the variable that is thought to either be affected or not is the dependent variable.

Researchers seek to determine whether or not their hypothesis, or hypotheses if they have more than one, will prove true. Sometimes they do, and sometimes they do not. Either way, the research is considered successful if one can conclude whether or not a hypothesis is true. 

Null Hypothesis

A researcher has a null hypothesis when she or he believes, based on theory and existing scientific evidence, that there will not be a relationship between two variables. For example, when examining what factors influence a person's highest level of education within the U.S., a researcher might expect that place of birth, number of siblings, and religion would not have an impact on the level of education. This would mean the researcher has stated three null hypotheses.

Alternative Hypothesis

Taking the same example, a researcher might expect that the economic class and educational attainment of one's parents, and the race of the person in question are likely to have an effect on one's educational attainment. Existing evidence and social theories that recognize the connections between wealth and cultural resources , and how race affects access to rights and resources in the U.S. , would suggest that both economic class and educational attainment of the one's parents would have a positive effect on educational attainment. In this case, economic class and educational attainment of one's parents are independent variables, and one's educational attainment is the dependent variable—it is hypothesized to be dependent on the other two.

Conversely, an informed researcher would expect that being a race other than white in the U.S. is likely to have a negative impact on a person's educational attainment. This would be characterized as a negative relationship, wherein being a person of color has a negative effect on one's educational attainment. In reality, this hypothesis proves true, with the exception of Asian Americans , who go to college at a higher rate than whites do. However, Blacks and Hispanics and Latinos are far less likely than whites and Asian Americans to go to college.

Formulating a Hypothesis

Formulating a hypothesis can take place at the very beginning of a research project , or after a bit of research has already been done. Sometimes a researcher knows right from the start which variables she is interested in studying, and she may already have a hunch about their relationships. Other times, a researcher may have an interest in ​a particular topic, trend, or phenomenon, but he may not know enough about it to identify variables or formulate a hypothesis.

Whenever a hypothesis is formulated, the most important thing is to be precise about what one's variables are, what the nature of the relationship between them might be, and how one can go about conducting a study of them.

Updated by Nicki Lisa Cole, Ph.D

  • Null Hypothesis Examples
  • Difference Between Independent and Dependent Variables
  • Examples of Independent and Dependent Variables
  • What Is a Hypothesis? (Science)
  • What Are the Elements of a Good Hypothesis?
  • Understanding Path Analysis
  • What It Means When a Variable Is Spurious
  • What 'Fail to Reject' Means in a Hypothesis Test
  • How Intervening Variables Work in Sociology
  • Null Hypothesis Definition and Examples
  • Scientific Method Vocabulary Terms
  • Understanding Simple vs Controlled Experiments
  • Null Hypothesis and Alternative Hypothesis
  • Six Steps of the Scientific Method
  • What Are Examples of a Hypothesis?
  • Scientific Method Flow Chart

Study.com

In order to continue enjoying our site, we ask that you confirm your identity as a human. Thank you very much for your cooperation.

Examples

AI Generator

formulation of hypothesis examples

When doing a research action plan students in school would know that the first thing to do is to know your topic well enough. From expecting science projects to work based on your predictions and the results that may have been quite the opposite from how you depicted them. This also rings true in businesses. There is a term for that and it is often associated with the subject Science, but can also be associated with business . Scientific method  or a hypothesis.

What Is a Hypothesis?

A hypothesis is a scientific wild guess, a prediction in research . A wild guess, a say from someone without any known proof.  A hypothesis can also mean a scientific, educated guess that most scientists and researchers do before planning out or doing experiments to check if their guesses or their scientific ideas based on their topics are exact or correct.

Hypothesis Format

A well-structured hypothesis is crucial for guiding scientific research. Here’s a detailed format for writing a hypothesis, along with examples for each step:

1. Start with a Research Question

Before writing a hypothesis, begin with a clear and concise research question . This question identifies the focus of your study.

Example Research Question: Does the amount of daily exercise affect weight loss?

2. Identify the Variables

Identify the independent and dependent variables in your research question.

  • Independent Variable: The variable you manipulate (e.g., amount of daily exercise).
  • Dependent Variable: The variable you measure (e.g., weight loss).

3. Formulate the Hypothesis

Use the identified variables to create a testable statement . This statement should clearly express the expected relationship between the variables.

  • If [independent variable], then [dependent variable].
  • [Independent variable] will [effect] [dependent variable].

Directional vs. Non-Directional Hypothesis:

  • Specifies the direction of the expected relationship.
  • Does not specify the direction of the expected relationship, only that a relationship exists.

4. Example Hypotheses Using the Format

Research question: does caffeine affect cognitive performance, if-then statement:.

  • Example: If individuals consume caffeine, then their cognitive performance will improve.

Direct Statement:

  • Example: Caffeine consumption will improve cognitive performance.

Null Hypothesis (H0):

  • Example: There is no significant effect of caffeine consumption on cognitive performance.

Alternative Hypothesis (H1):

  • Example: There is a significant effect of caffeine consumption on cognitive performance.

Directional Hypothesis:

Non-directional hypothesis:.

  • Example: There is a relationship between caffeine consumption and cognitive performance.

5. Refining the Hypothesis

Ensure that your hypothesis is specific, measurable, and testable. Avoid vague terms and focus on a single independent and dependent variable.

Hypothesis Examples in Research

A hypothesis is a statement that predicts the relationship between variables. It serves as a foundation for research by providing a clear focus and direction for experiments and data analysis . Here are examples of hypotheses from various fields of research:

Research Question:

Does sunlight exposure affect plant growth?

Hypotheses:

  • Null Hypothesis (H0): There is no significant difference in plant growth between plants exposed to sunlight and those kept in the shade.
  • Alternative Hypothesis (H1): Plants exposed to sunlight grow taller than those kept in the shade.
  • Directional Hypothesis: Increased sunlight exposure will lead to increased plant growth.
  • If-Then Statement: If plants are exposed to more sunlight, then they will grow taller.

2. Psychology

Does sleep duration affect memory retention?

  • Null Hypothesis (H0): There is no significant difference in memory retention between individuals who sleep for 8 hours and those who sleep for 4 hours.
  • Alternative Hypothesis (H1): Individuals who sleep for 8 hours will have better memory retention than those who sleep for 4 hours.
  • Directional Hypothesis: Longer sleep duration will improve memory retention.
  • If-Then Statement: If individuals sleep for 8 hours, then their memory retention will improve compared to those who sleep for 4 hours.

3. Education

Do interactive teaching methods improve student engagement?

  • Null Hypothesis (H0): There is no significant difference in student engagement between interactive teaching methods and traditional lecture-based methods.
  • Alternative Hypothesis (H1): Interactive teaching methods result in higher student engagement compared to traditional lecture-based methods.
  • Directional Hypothesis: Interactive teaching methods will increase student engagement.
  • If-Then Statement: If teachers use interactive teaching methods, then student engagement will increase.

4. Medicine

Does a new drug reduce blood pressure more effectively than the standard medication?

  • Null Hypothesis (H0): There is no significant difference in blood pressure reduction between the new drug and the standard medication.
  • Alternative Hypothesis (H1): The new drug reduces blood pressure more effectively than the standard medication.
  • Directional Hypothesis: The new drug will reduce blood pressure more than the standard medication.
  • If-Then Statement: If patients take the new drug, then their blood pressure will decrease more than if they take the standard medication.

5. Sociology

Does socioeconomic status affect access to higher education?

  • Null Hypothesis (H0): There is no significant relationship between socioeconomic status and access to higher education.
  • Alternative Hypothesis (H1): Higher socioeconomic status is associated with greater access to higher education.
  • Directional Hypothesis: Individuals with higher socioeconomic status will have greater access to higher education.
  • If-Then Statement: If individuals have a higher socioeconomic status, then they will have greater access to higher education.

Hypothesis Examples in Psychology

Psychology research often explores the relationships between various cognitive, behavioral, and emotional variables. Here are some well-structured hypothesis examples in psychology:

1. Sleep Duration and Memory Retention

  • Non-Directional Hypothesis: There is a relationship between sleep duration and memory retention.

2. Exercise and Anxiety Levels

Does regular exercise reduce anxiety levels?

  • Null Hypothesis (H0): There is no significant difference in anxiety levels between individuals who exercise regularly and those who do not.
  • Alternative Hypothesis (H1): Individuals who exercise regularly will have lower anxiety levels than those who do not.
  • Directional Hypothesis: Regular exercise will decrease anxiety levels.
  • Non-Directional Hypothesis: There is a relationship between regular exercise and anxiety levels.
  • If-Then Statement: If individuals exercise regularly, then their anxiety levels will decrease.

3. Social Media Usage and Self-Esteem

Does social media usage affect self-esteem in teenagers?

  • Null Hypothesis (H0): There is no significant relationship between social media usage and self-esteem in teenagers.
  • Alternative Hypothesis (H1): High social media usage is associated with lower self-esteem in teenagers.
  • Directional Hypothesis: Increased social media usage will decrease self-esteem in teenagers.
  • Non-Directional Hypothesis: There is a relationship between social media usage and self-esteem in teenagers.
  • If-Then Statement: If teenagers spend more time on social media, then their self-esteem will decrease.

4. Cognitive Behavioral Therapy (CBT) and Depression

Is Cognitive Behavioral Therapy (CBT) effective in reducing symptoms of depression?

  • Null Hypothesis (H0): There is no significant difference in depression symptoms between individuals who undergo CBT and those who do not.
  • Alternative Hypothesis (H1): Individuals who undergo CBT will experience a greater reduction in depression symptoms than those who do not.
  • Directional Hypothesis: CBT will reduce symptoms of depression.
  • Non-Directional Hypothesis: There is a relationship between undergoing CBT and reduction in depression symptoms.
  • If-Then Statement: If individuals undergo CBT, then their symptoms of depression will decrease.

5. Parental Involvement and Academic Achievement

Does parental involvement influence academic achievement in children?

  • Null Hypothesis (H0): There is no significant relationship between parental involvement and academic achievement in children.
  • Alternative Hypothesis (H1): Higher levels of parental involvement are associated with higher academic achievement in children.
  • Directional Hypothesis: Increased parental involvement will improve academic achievement in children.
  • Non-Directional Hypothesis: There is a relationship between parental involvement and academic achievement in children.
  • If-Then Statement: If parents are more involved in their children’s education, then their children will achieve higher academic success.

Hypothesis Examples in Science

Scientific research often involves creating hypotheses to test the relationships between variables. Here are some well-structured hypothesis examples from various fields of science:

1. Biology: Sunlight and Plant Growth

  • Non-Directional Hypothesis: There is a relationship between sunlight exposure and plant growth.

2. Chemistry: Temperature and Reaction Rate

Does temperature affect the rate of a chemical reaction?

  • Null Hypothesis (H0): There is no significant difference in the reaction rate of a chemical reaction at different temperatures.
  • Alternative Hypothesis (H1): Increasing the temperature will increase the reaction rate.
  • Directional Hypothesis: Higher temperatures will increase the reaction rate.
  • Non-Directional Hypothesis: There is a relationship between temperature and the reaction rate.
  • If-Then Statement: If the temperature of a reaction increases, then the reaction rate will increase.

3. Physics: Mass and Free Fall Speed

Does the mass of an object affect its speed when falling?

  • Null Hypothesis (H0): There is no significant difference in the falling speed of objects with different masses.
  • Alternative Hypothesis (H1): Objects with greater mass fall faster than those with lesser mass.
  • Directional Hypothesis: Heavier objects will fall faster than lighter objects.
  • Non-Directional Hypothesis: There is a relationship between the mass of an object and its falling speed.
  • If-Then Statement: If an object’s mass increases, then its falling speed will increase.

4. Environmental Science: Fertilizers and Water Quality

Do chemical fertilizers affect water quality in nearby lakes?

  • Null Hypothesis (H0): There is no significant effect of chemical fertilizers on the water quality of nearby lakes.
  • Alternative Hypothesis (H1): Chemical fertilizers negatively affect the water quality of nearby lakes.
  • Directional Hypothesis: The use of chemical fertilizers will decrease the water quality of nearby lakes.
  • Non-Directional Hypothesis: There is a relationship between the use of chemical fertilizers and the water quality of nearby lakes.
  • If-Then Statement: If chemical fertilizers are used, then the water quality in nearby lakes will decrease.

5. Earth Science: Soil Composition and Erosion Rate

Does soil composition affect the rate of erosion?

  • Null Hypothesis (H0): There is no significant difference in the erosion rate of soils with different compositions.
  • Alternative Hypothesis (H1): Soil composition affects the rate of erosion.
  • Directional Hypothesis: Soils with higher clay content will erode more slowly than sandy soils.
  • Non-Directional Hypothesis: There is a relationship between soil composition and the rate of erosion.
  • If-Then Statement: If soil has a higher clay content, then its erosion rate will be lower compared to sandy soil.

Hypothesis Examples in Biology

In biology, hypotheses are used to explore relationships and effects within biological systems. Here are some well-structured hypothesis examples in various areas of biology:

1. Photosynthesis and Light Intensity

How does light intensity affect the rate of photosynthesis in plants?

  • Null Hypothesis (H0): Light intensity has no significant effect on the rate of photosynthesis in plants.
  • Alternative Hypothesis (H1): Light intensity significantly affects the rate of photosynthesis in plants.
  • Directional Hypothesis: Increased light intensity will increase the rate of photosynthesis in plants.
  • Non-Directional Hypothesis: There is a relationship between light intensity and the rate of photosynthesis in plants.
  • If-Then Statement: If light intensity increases, then the rate of photosynthesis in plants will increase.

2. Temperature and Enzyme Activity

How does temperature affect the activity of the enzyme amylase?

  • Null Hypothesis (H0): Temperature has no significant effect on the activity of the enzyme amylase.
  • Alternative Hypothesis (H1): Temperature significantly affects the activity of the enzyme amylase.
  • Directional Hypothesis: Increasing the temperature will increase the activity of the enzyme amylase up to an optimal point, after which activity will decrease.
  • Non-Directional Hypothesis: There is a relationship between temperature and the activity of the enzyme amylase.
  • If-Then Statement: If the temperature increases, then the activity of the enzyme amylase will increase up to an optimal temperature, after which it will decrease.

3. Nutrient Availability and Plant Growth

Does the availability of nutrients in soil affect the growth of plants?

  • Null Hypothesis (H0): Nutrient availability has no significant effect on the growth of plants.
  • Alternative Hypothesis (H1): Nutrient availability significantly affects the growth of plants.
  • Directional Hypothesis: Increased nutrient availability will enhance plant growth.
  • Non-Directional Hypothesis: There is a relationship between nutrient availability and plant growth.
  • If-Then Statement: If nutrient availability in the soil increases, then the growth of plants will be enhanced.

4. Genetic Variation and Disease Resistance

Does genetic variation in a population affect its resistance to diseases?

  • Null Hypothesis (H0): Genetic variation has no significant effect on disease resistance in a population.
  • Alternative Hypothesis (H1): Genetic variation significantly affects disease resistance in a population.
  • Directional Hypothesis: Populations with greater genetic variation will have higher resistance to diseases.
  • Non-Directional Hypothesis: There is a relationship between genetic variation and disease resistance in a population.
  • If-Then Statement: If a population has greater genetic variation, then its resistance to diseases will be higher.

5. Water pH and Aquatic Life Health

Does the pH level of water affect the health of aquatic life?

  • Null Hypothesis (H0): The pH level of water has no significant effect on the health of aquatic life.
  • Alternative Hypothesis (H1): The pH level of water significantly affects the health of aquatic life.
  • Directional Hypothesis: Extreme pH levels (both high and low) will negatively affect the health of aquatic life.
  • Non-Directional Hypothesis: There is a relationship between the pH level of water and the health of aquatic life.
  • If-Then Statement: If the pH level of water is too high or too low, then the health of aquatic life will be negatively affected.

Hypothesis Examples in Sociology

In sociology, hypotheses are used to explore and explain social phenomena, behaviors, and relationships within societies. Here are some well-structured hypothesis examples in various areas of sociology:

1. Education and Social Mobility

Does access to higher education affect social mobility?

  • Null Hypothesis (H0): Access to higher education has no significant effect on social mobility.
  • Alternative Hypothesis (H1): Access to higher education significantly affects social mobility.
  • Directional Hypothesis: Increased access to higher education will improve social mobility.
  • Non-Directional Hypothesis: There is a relationship between access to higher education and social mobility.
  • If-Then Statement: If individuals have increased access to higher education, then their social mobility will improve.

2. Income Inequality and Crime Rates

Does income inequality influence crime rates in urban areas?

  • Null Hypothesis (H0): Income inequality has no significant effect on crime rates in urban areas.
  • Alternative Hypothesis (H1): Income inequality significantly affects crime rates in urban areas.
  • Directional Hypothesis: Higher income inequality will lead to higher crime rates in urban areas.
  • Non-Directional Hypothesis: There is a relationship between income inequality and crime rates in urban areas.
  • If-Then Statement: If income inequality increases, then crime rates in urban areas will increase.

3. Social Media Use and Social Interaction

Does the use of social media affect face-to-face social interactions among teenagers?

  • Null Hypothesis (H0): The use of social media has no significant effect on face-to-face social interactions among teenagers.
  • Alternative Hypothesis (H1): The use of social media significantly affects face-to-face social interactions among teenagers.
  • Directional Hypothesis: Increased use of social media will decrease face-to-face social interactions among teenagers.
  • Non-Directional Hypothesis: There is a relationship between the use of social media and face-to-face social interactions among teenagers.
  • If-Then Statement: If teenagers use social media more frequently, then their face-to-face social interactions will decrease.

4. Gender Roles and Career Choices

Do traditional gender roles influence career choices among young adults?

  • Null Hypothesis (H0): Traditional gender roles have no significant effect on career choices among young adults.
  • Alternative Hypothesis (H1): Traditional gender roles significantly affect career choices among young adults.
  • Directional Hypothesis: Adherence to traditional gender roles will limit career choices among young adults.
  • Non-Directional Hypothesis: There is a relationship between traditional gender roles and career choices among young adults.
  • If-Then Statement: If young adults adhere to traditional gender roles, then their career choices will be limited.

5. Cultural Diversity and Workplace Productivity

Does cultural diversity in the workplace affect productivity levels?

  • Null Hypothesis (H0): Cultural diversity in the workplace has no significant effect on productivity levels.
  • Alternative Hypothesis (H1): Cultural diversity in the workplace significantly affects productivity levels.
  • Directional Hypothesis: Increased cultural diversity will improve productivity levels in the workplace.
  • Non-Directional Hypothesis: There is a relationship between cultural diversity in the workplace and productivity levels.
  • If-Then Statement: If the workplace has increased cultural diversity, then productivity levels will improve.

More Hypothesis Samples & Examples in PDF

1. research hypothesis.

Research Hypothesis

2. Education Hypothesis

Education Hypothesis

3. Basic Hypothesis

Basic Hypothesis

4. Hypothesis Statement Template

Hypothesis Statement Template

5. Hypothesis in PDF

Hypothesis in PDF

6. Hypothesis Format

Hypothesis Format

7. Hypothesis Examples

Hypothesis Examples

8. Simple Hypothesis

Simple Hypothesis

Types of Hypothesis

Types of Hypothesis

A hypothesis is a statement that can be tested and is often used in scientific research to propose a relationship between two or more variables. Understanding the different types of hypotheses is essential for conducting effective research. Below are the main types of hypotheses:

1. Null Hypothesis (H0)

The null hypothesis states that there is no relationship between the variables being studied. It assumes that any observed effect is due to chance. Researchers often aim to disprove the null hypothesis.

Example: There is no significant difference in test scores between students who study with music and those who study in silence.

2. Alternative Hypothesis (H1 or Ha)

The alternative hypothesis suggests that there is a relationship between the variables being studied. It is what researchers seek to prove.

Example: Students who study with music have higher test scores than those who study in silence.

3. Simple Hypothesis

A simple hypothesis predicts a relationship between a single independent variable and a single dependent variable.

Example: Increasing the amount of sunlight will increase the growth rate of plants.

4. Complex Hypothesis

A complex hypothesis predicts a relationship involving two or more independent variables and/or two or more dependent variables.

Example: Increasing sunlight and water will increase the growth rate and height of plants.

5. Directional Hypothesis

A directional hypothesis specifies the direction of the expected relationship between variables. It suggests whether the relationship is positive or negative.

Example: Students who study for more hours will score higher on exams.

6. Non-Directional Hypothesis

A non-directional hypothesis does not specify the direction of the relationship. It only states that a relationship exists.

Example: There is a difference in test scores between students who study with music and those who study in silence.

7. Statistical Hypothesis

A statistical hypothesis involves quantitative data and can be tested using statistical methods. It often includes both null and alternative hypotheses.

Example: The mean test scores of students who study with music are significantly different from those who study in silence.

8. Causal Hypothesis

A causal hypothesis proposes a cause-and-effect relationship between variables. It suggests that one variable causes a change in another.

Example: Smoking causes lung cancer.

9. Associative Hypothesis

An associative hypothesis suggests that variables are related but does not imply causation.

Example: There is an association between physical activity levels and body weight.

10. Research Hypothesis

A research hypothesis is a broad statement that serves as the foundation for the research study. It is often the same as the alternative hypothesis.

Example: Implementing a new teaching strategy will improve student engagement and performance.

How To Use Hypothesis for Research?

A hypothesis is a critical component of the research process, providing a clear direction for the study and forming the basis for drawing conclusions. Here’s a step-by-step guide on how to use a hypothesis in research:

1. Identify the Research Problem

Before formulating a hypothesis, clearly define the research problem or question. This step involves understanding what you aim to investigate and why it is significant.

Example: You want to study the impact of sleep on academic performance among college students.

2. Review Existing Literature

Conduct a thorough review of existing literature to understand what is already known about the topic. This helps in identifying gaps in knowledge and forming a basis for your hypothesis.

Example: Previous studies suggest a positive correlation between sleep duration and academic performance but lack specific data on college students.

Based on the research problem and literature review, formulate a clear and testable hypothesis. Ensure it is specific and relates directly to the variables being studied.

Types of Hypotheses:

  • Null Hypothesis (H0): There is no significant relationship between sleep duration and academic performance among college students.
  • Alternative Hypothesis (H1): There is a significant relationship between sleep duration and academic performance among college students.

4. Define Variables

Clearly define the independent and dependent variables involved in the hypothesis.

  • Independent Variable: Sleep duration
  • Dependent Variable: Academic performance (e.g., GPA)

5. Design the Study

Choose an appropriate research design to test the hypothesis. This could be experimental, correlational, or observational, depending on the nature of your research question.

Example: Conduct a correlational study to examine the relationship between sleep duration and GPA among college students.

6. Collect Data

Gather data through surveys, experiments, or secondary data sources. Ensure the data collection methods are reliable and valid to accurately test the hypothesis.

Example: Use a questionnaire to collect data on students’ sleep duration and their GPAs.

7. Analyze the Data

Use appropriate statistical methods to analyze the data. This step involves testing the hypothesis to determine whether to accept or reject the null hypothesis.

Example: Perform a Pearson correlation analysis to examine the relationship between sleep duration and GPA.

8. Interpret the Results

Interpret the results of the statistical analysis. Determine if the data supports the alternative hypothesis or if the null hypothesis cannot be rejected.

Example: If the analysis shows a significant positive correlation, you can reject the null hypothesis and accept the alternative hypothesis that sleep duration is related to academic performance.

9. Draw Conclusions

Draw conclusions based on the results of the hypothesis testing. Discuss the implications of the findings and how they contribute to the existing body of knowledge.

Example: Conclude that longer sleep duration is associated with higher GPA among college students and discuss potential implications for student health and academic policies.

10. Report and Share Findings

Write a detailed report or research paper presenting the hypothesis, methodology, results, and conclusions. Share your findings with the academic community or relevant stakeholders.

Example: Publish the study in a peer-reviewed journal or present it at an academic conference.

How to Write a Hypothesis?

Writing a hypothesis is a crucial step in the scientific method. A well-constructed hypothesis guides your research, helping you design experiments and analyze results. Here’s a step-by-step guide on how to write an effective hypothesis:

1. Understand the Research Question

Start by clearly understanding the research question or problem you want to address. This helps in formulating a focused hypothesis.

Example: How does sunlight exposure affect plant growth?

2. Conduct Preliminary Research

Review existing literature related to your research question. This helps in understanding what is already known and identifying gaps in knowledge.

Example: Studies show that plants generally grow better with more sunlight, but the optimal amount varies.

3. Identify Variables

Determine the independent and dependent variables for your study.

  • Independent Variable: The factor you manipulate (e.g., sunlight exposure).
  • Dependent Variable: The factor you measure (e.g., plant growth).

4. Formulate a Simple Hypothesis

A simple hypothesis involves one independent and one dependent variable. Clearly state the expected relationship between these variables.

Example: Increasing sunlight exposure will increase plant growth.

5. Choose the Type of Hypothesis

Decide whether your hypothesis will be null or alternative, directional or non-directional.

  • Null Hypothesis (H0): There is no relationship between the variables.
  • Alternative Hypothesis (H1): There is a relationship between the variables.
  • Directional Hypothesis: Specifies the direction of the relationship.
  • Non-Directional Hypothesis: Does not specify the direction.

Example of Directional Hypothesis: Plants exposed to more sunlight will grow taller than those exposed to less sunlight.

6. Ensure Testability

Make sure your hypothesis can be tested through experiments or observations. It should be measurable and falsifiable.

Example: Plants will be grown under different levels of sunlight, and their growth will be measured over time.

7. Write the Hypothesis

Write your hypothesis in a clear, concise, and specific manner. It should include the variables and the expected relationship between them.

Example: If plants are exposed to increased sunlight, then they will grow taller compared to plants that receive less sunlight.

8. Refine the Hypothesis

Ensure that your hypothesis is specific and narrow enough to be testable but broad enough to cover the scope of your research.

Example: If tomato plants are exposed to 8 hours of sunlight per day, then they will grow taller and produce more fruit compared to tomato plants exposed to 4 hours of sunlight per day.

How Do You Formulate a Hypothesis?

To formulate a hypothesis, identify the research question, review existing literature, define variables, and create a testable statement predicting the relationship between the variables.

What Is the Difference Between Null and Alternative Hypotheses?

The null hypothesis (H0) states there is no effect or relationship, while the alternative hypothesis (H1) proposes that there is an effect or relationship.

Why Is a Hypothesis Important in Research?

A hypothesis provides a clear focus for the study, guiding the research design, data collection, and analysis, ultimately helping to draw meaningful conclusions.

Can a Hypothesis Be Proven True?

A hypothesis cannot be proven true; it can only be supported or refuted through experimentation and analysis. Even if supported, it remains open to further testing.

What Makes a Good Hypothesis?

A good hypothesis is clear, concise, specific, testable, and based on existing knowledge. It should predict a relationship between variables that can be measured.

How Is a Hypothesis Tested?

A hypothesis is tested through experiments or observations, collecting and analyzing data to determine if the results support or refute the hypothesis.

What Are the Types of Hypotheses?

Types of hypotheses include null, alternative, simple, complex, directional, non-directional, statistical, causal, and associative.

What Is a Directional Hypothesis?

A directional hypothesis specifies the expected direction of the relationship between variables, indicating whether the effect will be positive or negative.

What Is a Non-Directional Hypothesis?

A non-directional hypothesis states that a relationship exists between variables but does not specify the direction of the relationship.

How Do You Refine a Hypothesis?

Refine a hypothesis by ensuring it is specific, measurable, and testable. Remove any vague terms and focus on a single independent and dependent variable.

Twitter

Text prompt

  • Instructive
  • Professional

10 Examples of Public speaking

20 Examples of Gas lighting

Find Study Materials for

  • Explanations
  • Business Studies
  • Combined Science
  • Computer Science
  • Engineering
  • English literature
  • Environmental Science
  • Human Geography
  • Macroeconomics
  • Microeconomics
  • Social Studies
  • Browse all subjects
  • Textbook Solutions
  • Read our Magazine

Create Study Materials

  • Flashcards Create and find the best flashcards.
  • Notes Create notes faster than ever before.
  • Study Sets Everything you need for your studies in one place.
  • Study Plans Stop procrastinating with our smart planner features.
  • Formulation of Hypothesis

Children who spend more time playing outside are more likely to be imaginative. What do you think this statement is an example of in terms of scientific research ? If you guessed a hypothesis, then you'd be correct. The formulation of hypotheses is a fundamental step in psychology research.

Formulation of Hypothesis

Create learning materials about Formulation of Hypothesis with our free learning app!

  • Instand access to millions of learning materials
  • Flashcards, notes, mock-exams and more
  • Everything you need to ace your exams
  • Approaches in Psychology
  • Basic Psychology
  • Biological Bases of Behavior
  • Biopsychology
  • Careers in Psychology
  • Clinical Psychology
  • Bartlett War of the Ghosts
  • Brain Development
  • Bruner and Minturn Study of Perceptual Set
  • Case Studies Psychology
  • Computation
  • Conservation of Number Piaget
  • Constructive Processes in Memory
  • Correlation
  • Data handling
  • Depth Cues Psychology
  • Designing Research
  • Developmental Research
  • Dweck's Theory of Mindset
  • Ethical considerations in research
  • Experimental Method
  • Factors Affecting Perception
  • Factors Affecting the Accuracy of Memory
  • Gibson's Theory of Direct Perception
  • Gregory's Constructivist Theory of Perception
  • Gunderson et al 2013 study
  • Hughes Policeman Doll Study
  • Issues and Debates in Developmental Psychology
  • Language and Perception
  • McGarrigle and Donaldson Naughty Teddy
  • Memory Processes
  • Memory recall
  • Nature and Nurture in Development
  • Normal Distribution Psychology
  • Perception Research
  • Perceptual Set
  • Piagets Theory in Education
  • Planning and Conducting Research
  • Population Samples
  • Primary and Secondary Data
  • Quantitative Data
  • Quantitative and Qualitative Data
  • Quantitative and Qualitative Methods
  • Research Procedures
  • Serial Position Effect
  • Short-term Retention
  • Structures of Memory
  • Tables, Charts and Graphs
  • The Effects of Learning on Development
  • The Gilchrist And Nesberg Study Of Motivation
  • Three Mountains Task
  • Types of Variable
  • Types of bias and how to control
  • Visual Cues and Constancies
  • Visual illusions
  • Willingham's Learning Theory
  • Cognition and Development
  • Cognitive Psychology
  • Data Handling and Analysis
  • Developmental Psychology
  • Eating Behaviour
  • Emotion and Motivation
  • Famous Psychologists
  • Forensic Psychology
  • Health Psychology
  • Individual Differences Psychology
  • Issues and Debates in Psychology
  • Personality in Psychology
  • Psychological Treatment
  • Relationships
  • Research Methods in Psychology
  • Schizophrenia
  • Scientific Foundations of Psychology
  • Scientific Investigation
  • Sensation and Perception
  • Social Context of Behaviour
  • Social Psychology
  • First, we will discuss the importance of hypotheses in research.
  • We will then cover formulating hypotheses in research, including the steps in the formulation of hypotheses in research methodology.
  • We will provide examples of hypotheses in research throughout the explanation.
  • Finally, we will delve into the different types of hypotheses in research.

What is a Hypothesis?

The current community of psychologists believe that the best approach to understanding behaviour is to conduct scientific research . To be classed as scientific research , it must be observable, valid, reliable and follow a standardised procedure.

One of the important steps in scientific research is to formulate a hypothesis before starting the study procedure.

The hypothesis is a predictive, testable statement predicting the outcome and the results the researcher expects to find.

The hypothesis provides a summary of what direction, if any, is taken to investigate a theory.

In scientific research, there is a criterion that hypotheses need to be met to be regarded as acceptable.

If a hypothesis is disregarded, the research may be rejected by the community of psychology researchers.

Importance of Hypothesis in Research

The purpose of including hypotheses in psychology research is:

  • To provide a summary of the research, how it will be investigated, and what is expected to be found.
  • To provide an answer to the research question.

When carrying out research, researchers first investigate the research area they are interested in. From this, researchers are required to identify a gap in the literature.

Filling the gap essentially means finding what previous work has not been explained yet, investigated to a sufficient degree, or simply expanding or further investigating a theory if doubt exists.

The researcher then forms a research question that the researcher will attempt to answer in their study.

Remember, the hypothesis is a predictive statement of what is expected to happen when testing the research question.

The hypothesis can be used for later data analysis. This includes inferential tests such as hypothesis testing and identifying if statistical findings are significant.

Formulation of testable hypotheses, four people with question marks above their heads, Vaia

Steps in the Formulation of Hypothesis in Research Methodology

Researchers must follow certain steps to formulate testable hypotheses when conducting research.

Overall, the researcher has to consider the direction of the research, i.e. will it be looking for a difference caused by independent variables ? Or will it be more concerned with the correlation between variables?

All researchers will likely complete the following.

  • Investigating background research in the area of interest.
  • Formulating or investigating a theory.
  • Identify how the theory will be tested and what the researcher expects to find based on relevant, previously published scientific works.

The above steps are used to formulate testable hypotheses.

The Formulation of Testable Hypotheses

The hypothesis is important in research as it indicates what and how a variable will be investigated.

The hypothesis essentially summarises what and how something will be investigated. This is important as it ensures that the researcher has carefully planned how the research will be done, as the researchers have to follow a set procedure to conduct research.

This is known as the scientific method.

Formulating Hypotheses in Research

When formulating hypotheses, things that researchers should consider are:

Hypothesis RequirementDescription
It should be written as predictive statements regarding the relationship between the IV and DV.The researcher should be able to predict what they expect to find from the study results. The researcher could state that they expect to see a difference. Occasionally, researchers may theorise what changes are expected to be observed (two-tailed alternative hypothesis).
It should be formulated based on background research.Hypotheses should not be based on guesswork. Instead, researchers should use previously published research to predict the study's expected outcome.
Identify the IV. IV is what the experimenter manipulates to see if it affects the DV.
Identify the DV.DV is the variable being measured after the IV has been manipulated or after it changes during the experiment.
The should be operationalised. The researchers must define how each variable (IV and DV) will be measured. For example, may be measured using a performance test, such as the Mini-Mental Status Examination. When a hypothesis is operationalised, it is testable.
The hypotheses need to be falsifiable.Other researchers need to be able to replicate the research using the same variables to see whether they can verify the results. The hypothesis needs to be written in a way that is falsifiable, meaning it can be tested using the scientific method to see if it is true.An example of a non-falsifiable hypothesis is "leprechauns always find the pot of gold at the end of the rainbow."
The hypotheses should be clear. Hypotheses are usually only a sentence long and should only include the details summarised above. A good hypothesis should not include irrelevant information.

Types of Hypotheses in Research

Researchers can propose different types of hypotheses when carrying out research.

The following research scenario will be discussed to show examples of each type of hypothesis that the researchers could use. "A research team was investigating whether memory performance is affected by depression ."

The identified independent variable is the severity of depression scores, and the dependent variable is the scores from a memory performance task.

The null hypothesis predicts that the results will show no or little effect. The null hypothesis is a predictive statement that researchers use when it is thought that the IV will not influence the DV.

In this case, the null hypothesis would be there will be no difference in memory scores on the MMSE test of those who are diagnosed with depression and those who are not.

An alternative hypothesis is a predictive statement used when it is thought that the IV will influence the DV. The alternative hypothesis is also called a non-directional, two-tailed hypothesis, as it predicts the results can go either way, e.g. increase or decrease.

The example in this scenario is there will be an observed difference in scores from a memory performance task between people with high- or low-depressive scores.

The directional alternative hypothesis states how the IV will influence the DV, identifying a specific direction, such as if there will be an increase or decrease in the observed results.

The example in this scenario is people with low depressive scores will perform better in the memory performance task than people who score higher in depressive symptoms.

Example Hypothesis in Research

To summarise, let's look at an example of a straightforward hypothesis that indicates the relationship between two variables: the independent and the dependent.

If you stay up late, you will feel tired the following day; the more caffeine you drink, the harder you find it to fall asleep, or the more sunlight plants get, the taller they will grow.

Formulation of Hypothesis - Key Takeaways

  • The current community of psychologists believe that the best approach to understanding behaviour is to conduct scientific research. One of the important steps in scientific research is to create a hypothesis.
  • The hypothesis is a predictive, testable statement concerning the outcome/results that the researcher expects to find.
  • Hypotheses are needed in research to provide a summary of what the research is, how to investigate a theory and what is expected to be found, and to provide an answer to the research question so that the hypothesis can be used for later data analysis.
  • There are requirements for the formulation of testable hypotheses. The hypotheses should identify and operationalise the IV and DV. In addition, they should describe the nature of the relationship between the IV and DV.
  • There are different types of hypotheses: Null hypothesis, Alternative hypothesis (this is also known as the non-directional, two-tailed hypothesis), and Directional hypothesis (this is also known as the one-tailed hypothesis).

Flashcards in Formulation of Hypothesis 18

What type of hypothesis matches the following definition. A predictive statement that researchers use when it is thought that the IV will not influence the DV.

Null hypothesis 

What type of hypothesis matches the following definition. A hypothesis that states that the IV will influence the DV. But, the hypothesis does not state how the IV will influence the DV. 

Alternative hypothesis 

What type of hypothesis matches the following definition. A hypothesis that states that the IV will influence the DV, and states how it will influence the DV. 

Directional, alternative hypothesis 

Which type of hypothesis is also known as a two-tailed hypothesis? 

What type of hypothesis is the following example. There will be no observed difference in scores from a memory performance task between people with high- or low-depressive scores.

What type of hypothesis is the following example. There will be an observed difference in scores from a memory performance task between people with high- or low-depressive scores.

Formulation of Hypothesis

Learn with 18 Formulation of Hypothesis flashcards in the free Vaia app

We have 14,000 flashcards about Dynamic Landscapes.

Already have an account? Log in

Frequently Asked Questions about Formulation of Hypothesis

What are the 3 types of hypotheses?

The three types of hypotheses are:

  • Null hypothesis 
  • Alternative hypothesis 
  • Directional/non-directional hypothesis 

What is an example of a hypothesis in psychology?

An example of a null hypothesis in psychology is, there will be no observed difference in scores from a memory performance task between people with high- or low-depressive scores.

What are the steps in formulating a hypothesis?

All researchers will likely complete the following

  • Investigating background research in the area of interest 
  • Formulating or investigating a theory 
  • Identify how the theory will be tested and what the researcher expects to find based on relevant, previously published scientific works 

What is formulation of hypothesis in research? 

The formulation of a hypothesis in research is when the researcher formulates a predictive statement of what is expected to happen when testing the research question based on background research.

How to formulate  null and alternative hypothesis?

When formulating a null hypothesis the researcher would state a prediction that they expect to see no difference in the dependent variable when the independent variable changes or is manipulated. Whereas, when using an alternative hypothesis then it would be predicted that there will be a change in the dependent variable. The researcher can state in which direction they expect the results to go. 

Test your knowledge with multiple choice flashcards

Formulation of Hypothesis

Join the Vaia App and learn efficiently with millions of flashcards and more!

Keep learning, you are doing great.

Discover learning materials with the free Vaia app

1

Vaia is a globally recognized educational technology company, offering a holistic learning platform designed for students of all ages and educational levels. Our platform provides learning support for a wide range of subjects, including STEM, Social Sciences, and Languages and also helps students to successfully master various tests and exams worldwide, such as GCSE, A Level, SAT, ACT, Abitur, and more. We offer an extensive library of learning materials, including interactive flashcards, comprehensive textbook solutions, and detailed explanations. The cutting-edge technology and tools we provide help students create their own learning materials. StudySmarter’s content is not only expert-verified but also regularly updated to ensure accuracy and relevance.

Formulation of Hypothesis

Vaia Editorial Team

Team Psychology Teachers

  • 8 minutes reading time
  • Checked by Vaia Editorial Team

Study anywhere. Anytime.Across all devices.

Create a free account to save this explanation..

Save explanations to your personalised space and access them anytime, anywhere!

By signing up, you agree to the Terms and Conditions and the Privacy Policy of Vaia.

Sign up to highlight and take notes. It’s 100% free.

Join over 22 million students in learning with our Vaia App

The first learning app that truly has everything you need to ace your exams in one place

  • Flashcards & Quizzes
  • AI Study Assistant
  • Study Planner
  • Smart Note-Taking

Join over 22 million students in learning with our Vaia App

Privacy Overview

Get unlimited access with a free vaia account..

  • Instant access to millions of learning materials.
  • Flashcards, notes, mock-exams, AI tools and more.
  • Everything you need to ace your exams.

Second Popup Banner

Information

  • Author Services

Initiatives

You are accessing a machine-readable page. In order to be human-readable, please install an RSS reader.

All articles published by MDPI are made immediately available worldwide under an open access license. No special permission is required to reuse all or part of the article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. For more information, please refer to https://www.mdpi.com/openaccess .

Feature papers represent the most advanced research with significant potential for high impact in the field. A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications.

Feature papers are submitted upon individual invitation or recommendation by the scientific editors and must receive positive feedback from the reviewers.

Editor’s Choice articles are based on recommendations by the scientific editors of MDPI journals from around the world. Editors select a small number of articles recently published in the journal that they believe will be particularly interesting to readers, or important in the respective research area. The aim is to provide a snapshot of some of the most exciting work published in the various research areas of the journal.

Original Submission Date Received: .

  • Active Journals
  • Find a Journal
  • Proceedings Series
  • For Authors
  • For Reviewers
  • For Editors
  • For Librarians
  • For Publishers
  • For Societies
  • For Conference Organizers
  • Open Access Policy
  • Institutional Open Access Program
  • Special Issues Guidelines
  • Editorial Process
  • Research and Publication Ethics
  • Article Processing Charges
  • Testimonials
  • Preprints.org
  • SciProfiles
  • Encyclopedia

applsci-logo

Article Menu

formulation of hypothesis examples

  • Subscribe SciFeed
  • Recommended Articles
  • Author Biographies
  • Google Scholar
  • on Google Scholar
  • Table of Contents

Find support for a specific problem in the support section of our website.

Please let us know what you think of our products and services.

Visit our dedicated information section to learn more about MDPI.

JSmol Viewer

Advancements in fish vaccination: current innovations and future horizons in aquaculture health management.

formulation of hypothesis examples

1. Introduction

2. fish immune system, 3. bacterial, viral, and parasitic diseases in fish, 4. current licensed vaccines for bacterial diseases, 4.1. edwardsiellosis in fish, 4.2. enteric septicemia of catfish, 4.3. bacterial kidney disease, 4.4. flavobacteriosis/columnaris disease, 4.5. furunculosis, 4.6. piscine streptococcosis, 4.7. enteric red mouth disease/yersiniosis, 4.8. lactococcosis, 5. current licensed vaccines for viral diseases, 5.1. current licensed vaccines for parasitic diseases, 5.2. challenges and limitations in developing vaccines for fish, 6. summary and conclusions, conflicts of interest.

  • Adams, A. Progress, challenges and opportunities in fish vaccine development. Fish Shellfish. Immunol. 2019 , 90 , 210–214. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Wanja, D.W.; Mbuthia, P.G.; Waruiru, R.M.; Mwadime, J.M.; Bebora, L.C.; Nyaga, P.N.; Ngowi, H.A. Fish Husbandry Practices and Water Quality in Central Kenya: Potential Risk Factors for Fish Mortality and Infectious Diseases. Vet. Med. Int. 2020 , 2020 , 6839354. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Dhar, A.K.; Manna, S.K.; Allnutt, F.C.T. Viral vaccines for farmed finfish. Virusdisease 2014 , 25 , 1–17. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Surachetpong, W.; Roy, S.R.K.; Nicholson, P. Tilapia lake virus: The story so far. J. Fish Dis. 2020 , 43 , 1115–1132. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • FAO. Fisheries, Aquaculture Department. In The State of World Fisheries and Aquaculture ; FAO: Rome, Italy, 2012; pp. 1–153. [ Google Scholar ]
  • Assefa, A.; Abunna, F. Maintenance of Fish Health in Aquaculture: Review of Epidemiological Approaches for Prevention and Control of Infectious Disease of Fish. Vet. Med. Int. 2018 , 2018 , 5432497. [ Google Scholar ] [ CrossRef ]
  • Broughton, E.I.; Walker, D.G. Policies and practices for aquaculture food safety in China. Food Policy 2010 , 35 , 471–478. [ Google Scholar ] [ CrossRef ]
  • Rijkers, G.T.; Teunissen, A.G.; Van Oosterom, R.; Van Muiswinkel, W.B. The immune system of cyprinid fish. The immunosuppressive effect of the antibiotic oxytetracycline in carp ( Cyprinus carpio L.). Aquaculture 1980 , 19 , 177–189. [ Google Scholar ]
  • Salam, M.A.; Al-Amin, M.Y.; Salam, M.T.; Pawar, J.S.; Akhter, N.; Rabaan, A.A.; Alqumber, M.A.A. Antimicrobial Resistance: A Growing Serious Threat for Global Public Health. Healthcare 2023 , 11 , 1946. [ Google Scholar ] [ CrossRef ]
  • Ma, J.; Bruce, T.J.; Jones, E.M.; Cain, K.D. A Review of Fish Vaccine Development Strategies: Conventional Methods and Modern Biotechnological Approaches. Microorganisms 2019 , 7 , 569. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Horzinek, M.; Schijns, V.; Denis, M.; Desmettre, P.; Babiuk, L. General Description of Vaccines, Veterinary Vaccinology ; Elsevier: Amsterdam, The Netherlands, 1997; pp. 131–152. [ Google Scholar ]
  • Aquaculture, V.F.; USDA. AMS, LPS Agricultural Analytics Division for the USDA National Organic Program, USDA, 24 January 2014. Vaccines for Aquaculture (usda.gov).
  • Du, Y.; Hu, X.; Miao, L.; Chen, J. Current status and development prospects of aquatic vaccines. Front. Immunol. 2022 , 13 , 1040336. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Guo, M.; Li, C. An overview of cytokine used as adjuvants in fish: Current state and future trends. Rev. Aquac. 2021 , 13 , 996–1014. [ Google Scholar ] [ CrossRef ]
  • Secombes, C. Will advances in fish immunology change vaccination strategies? Fish Shellfish Immunol. 2008 , 25 , 409–416. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Langevin, C.; Aleksejeva, E.; Passoni, G.; Palha, N.; Levraud, J.-P.; Boudinot, P. The Antiviral Innate Immune Response in Fish: Evolution and Conservation of the IFN System. J. Mol. Biol. 2013 , 425 , 4904–4920. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Verrier, E.R.; Langevin, C.; Tohry, C.; Houel, A.; Ducrocq, V.; Benmansour, A.; Quillet, E.; Boudinot, P. Genetic resistance to rhabdovirus infection in teleost fish is paralleled to the derived cell resistance status. PLoS ONE 2012 , 7 , e33935. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Swain, B.; Powell, C.T.; Curtiss, R. Virulence, immunogenicity and live vaccine potential of aroA and phoP mutants of Edwardsiella piscicida in zebrafish. Microb. Pathog. 2022 , 162 , 105355. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Swain, B.; Powell, C.T.; Curtiss, R. Pathogenicity and immunogenicity of Edwardsiella piscicida ferric uptake regulator (fur) mutations in zebrafish. Fish Shellfish Immunol. 2020 , 107 , 497–510. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Swain, B.; Campodonico, V.A.; Curtiss, R. Recombinant Attenuated Edwardsiella piscicida Vaccine Displaying Regulated Lysis to Confer Biological Containment and Protect Catfish against Edwardsiellosis. Vaccines 2023 , 11 , 1470. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Shoemaker, C.A.; Klesius, P.H.; Evans, J.J.; Arias, C.R. Use of Modified Live Vaccines in Aquaculture. J. World Aquac. Soc. 2009 , 40 , 573–585. [ Google Scholar ] [ CrossRef ]
  • Gudding, R.; Van Muiswinkel, W.B. A history of fish vaccination: Science-based disease prevention in aquaculture. Fish Shellfish Immunol. 2013 , 35 , 1683–1688. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Swain, B.; Powell, C.T.; Curtiss, R. Construction and Evaluation of Recombinant Attenuated Edwardsiella piscicida Vaccine (RAEV) Vector System Encoding Ichthyophthirius multifiliis (Ich) Antigen IAG52B. Front. Immunol. 2022 , 12 , 802760. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Mondal, H.; Thomas, J. A review on the recent advances and application of vaccines against fish pathogens in aquaculture. Aquac. Int. J. Eur. Aquac. Soc. 2022 , 30 , 1971–2000. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Rauta, P.R.; Nayak, B.; Das, S. Immune system and immune responses in fish and their role in comparative immunity study: A model for higher organisms. Immunol. Lett. 2012 , 148 , 23–33. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Smith, N.C.; Rise, M.L.; Christian, S.L. A Comparison of the Innate and Adaptive Immune Systems in Cartilaginous Fish, Ray-Finned Fish, and Lobe-Finned Fish. Front. Immunol. 2019 , 10 , 2292. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Aristizábal, B.G.Á. Innate immune system. In Autoimmunity: From Bench to Bedside ; El Rosario University Press: Bogota, Colombia, 2013; Chapter 2. [ Google Scholar ]
  • Magnadóttir, B. Innate immunity of fish (overview). Fish Shellfish Immunol. 2006 , 20 , 137–151. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Mutoloki, S.; Munang, H.M.; Evensen, Ø. Oral Vaccination of Fish—Antigen Preparations, Uptake, and Immune Induction. Front. Immunol. 2015 , 6 , 519. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Swain, B.; Samanta, M.; Basu, M.; Panda, P.; Sahoo, B.R.; Maiti, N.K.; Mishra, B.K.; Eknath, A.E. Molecular characterization, inductive expression and mechanism of interleukin-10 gene induction in the Indian major carp, catla ( Catla catla ). Aquac. Res. 2012 , 43 , 897–907. [ Google Scholar ]
  • Janeway, C.A.; Travers, P.; Walport, M.; Capra, D.J. Immunobiology , 5th ed.; Garland Science: London, UK; Taylor & Francis Group: Abingdon, UK, 2001. [ Google Scholar ]
  • Storni, T.; Kündig, T.M.; Senti, G.; Johansen, P. Immunity in response to particulate antigen-delivery systems. Adv. Drug Deliv. Rev. 2005 , 57 , 333–355. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Jones, M.; Fosbery, R.; Gregory, J.; Taylor, D. Cambridge International AS and A Level Biology Coursebook with CD-ROM ; Cambridge University Press: Cambridge, UK, 2014. [ Google Scholar ]
  • Nakanishi, T.; Shibasaki, Y.; Matsuura, Y. T Cells in Fish. Biology 2015 , 4 , 640–663. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Yamasaki, M.; Araki, K.; Nakanishi, T.; Nakayasu, C.; Yoshiura, Y.; Iida, T.; Yamamoto, A. Adaptive immune response to Edwardsiella tarda infection in ginbuna crucian carp, Carassius auratus langsdorfii. Vet. Immunol. Immunopathol. 2013 , 153 , 83–90. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Wang, Q.; Ji, W.; Xu, Z. Current use and development of fish vaccines in China. Fish Shellfish Immunol. 2020 , 96 , 223–234. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Guo, Y.; Chen, Y.; Liu, X.; Min, J.-J.; Tan, W.; Zheng, J.H. Targeted cancer immunotherapy with genetically engineered oncolytic Salmonella typhimurium. Cancer Lett. 2020 , 469 , 102–110. [ Google Scholar ] [ CrossRef ]
  • Wamala, S.P.; Mugimba, K.K.; Mutoloki, S.; Evensen, Ø.; Mdegela, R.; Byarugaba, D.K.; Sørum, H. Occurrence and antibiotic susceptibility of fish bacteria isolated from Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish) in Uganda. Fish. Aquat. Sci. 2018 , 21 , 6. [ Google Scholar ] [ CrossRef ]
  • Viršek, M.K.; Lovšin, M.N.; Koren, Š.; Kržan, A.; Peterlin, M. Microplastics as a vector for the transport of the bacterial fish pathogen species Aeromonas salmonicida . Mar. Pollut. Bull. 2017 , 125 , 301–309. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Kumari, S. Freshwater and marine water fish diseases: A review. Int. J. Fish Aquat. Stud. 2020 , 8 , 4. [ Google Scholar ]
  • Gui, L.; Chinchar, V.G.; Zhang, Q. Molecular basis of pathogenesis of emerging viruses infecting aquatic animals. Aquac. Fish. 2018 , 3 , 1–5. [ Google Scholar ] [ CrossRef ]
  • Leong, J.C. Fish Viruses. Encycl. Virol. 2008 , 227–234. [ Google Scholar ] [ CrossRef ]
  • de las Heras, A.I.; Saint-Jean, S.R.; Pérez-Prieto, S.I. Immunogenic and protective effects of an oral DNA vaccine against infectious pancreatic necrosis virus in fish. Fish Shellfish Immunol. 2010 , 28 , 562–570. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Hølvold, L.B.; Myhr, A.I.; Dalmo, R.A. Strategies and hurdles using DNA vaccines to fish. Vet. Res. 2014 , 45 , 21. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Athanasopoulou, F.; Pappas, I.; Bitchava, K. An overview of the treatments of parasitic disease in Mediterranean aquaculture. Options Méditerranéennes 2009 , 86 , 65–83. [ Google Scholar ]
  • Francis-Floyd, R.Y.R.; Pouder, D. Ichthyophthirius multifiliis (White Spot) Infections in Fish ; Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida: Gainesville, FL, USA, 2018. [ Google Scholar ]
  • Bridging America and Russia with Shared Perspectives on Aquatic Animal Health: Proceedings of the Third Bilateral Conference between the United States and Russia, Aquatic Animal Health 2009 ; Khaled bin Sultan Living Oceans Foundation: Landover, MD, USA, 2011.
  • Toranzo, A.E.; Romalde, J.L.; Magariños, B.; Barja, J.L. Present and future of aquaculture vaccines against fish bacterial diseases. In The Use of Veterinary Drugs and Vaccines in Mediterranean Aquaculture ; Basurco, B., Rogers, C., Eds.; CIHEAM: Zaragoza, Spain, 2009; pp. 155–176. [ Google Scholar ]
  • Bujan, N.; Toranzo, A.; Magarinos, B. Edwardsiella piscicida : A significant bacterial pathogen of cultured fish. Dis. Aquat. Org. 2018 , 131 , 59–71. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Leung, K.Y.; Wang, Q.; Yang, Z.; Siame, B.A. Edwardsiella piscicida : A versatile emerging pathogen of fish. Virulence 2019 , 10 , 555–567. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Park, S.B.; Aoki, T.; Jung, T.S. Pathogenesis of and strategies for preventing Edwardsiella tarda infection in fish. Vet. Res. 2012 , 43 , 67. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Sayed, M.; Ozdemir, O.; Essa, M.; Olivier, A.; Karsi, A.; Lawrence, M.L.; Abdelhamed, H. Virulence and live vaccine potential of Edwardsiella piscicida phoP and phoQ mutants in catfish against edwardsiellosis. J. Fish Dis. 2021 , 44 , 1463–1474. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Yang, W.; Wang, L.; Zhang, L.; Qu, J.; Wang, Q.; Zhang, Y. An invasive and low virulent Edwardsiella tarda esrB mutant promising as live attenuated vaccine in aquaculture. Appl. Microbiol. Biotechnol. 2015 , 99 , 1765–1777. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Xi-zhi, J.G.; Dash, P.; Crawford, J.C.; Allen, E.K.; Zamora, A.E.; Boyd, D.F.; Duan, S.; Bajracharya, R.; Awad, W.A.; Apiwattanakul, N. Lung γδ T cells mediate protective responses during neonatal influenza infection that are associated with type 2 immunity. Immunity 2018 , 49 , 531–544.e6. [ Google Scholar ]
  • Zou, J.; Secombes, C.J. The Function of Fish Cytokines. Biology 2016 , 5 , 23. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Kordon, A.O.; Abdelhamed, H.; Ahmed, H.; Baumgartner, W.; Karsi, A.; Pinchuk, L.M. Assessment of the Live Attenuated and Wild-Type Edwardsiella ictaluri -Induced Immune Gene Expression and Langerhans-Like Cell Profiles in the Immune-Related Organs of Catfish. Front. Immunol. 2019 , 10 , 392. [ Google Scholar ] [ CrossRef ]
  • Abdelhamed, H.; Lawrence, M.L.; Karsi, A. Development and Characterization of a Novel Live Attenuated Vaccine against Enteric Septicemia of Catfish. Front. Microbiol. 2018 , 9 , 1819. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Wise, D.J.; Greenway, T.E.; Byars, T.S.; Griffin, M.J.; Khoo, L.H. Oral Vaccination of Channel Catfish against Enteric Septicemia of Catfish Using a Live Attenuated Edwardsiella ictaluri Isolate. J. Aquat. Anim. Health 2015 , 27 , 135–143. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Dahal, N.; Abdelhamed, H.; Lu, J.; Karsi, A.; Lawrence, M.L. Tricarboxylic Acid Cycle and One-Carbon Metabolism Pathways Are Important in Edwardsiella ictaluri Virulence. PLoS ONE 2013 , 8 , e65973. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Nho, S.W.; Abdelhamed, H.; Karsi, A.; Lawrence, M.L. Improving safety of a live attenuated Edwardsiella ictaluri vaccine against enteric septicemia of catfish and evaluation of efficacy. Vet. Microbiol. 2017 , 210 , 83–90. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Delghandi, M.R.; El-Matbouli, M.; Menanteau-Ledouble, S. Renibacterium salmoninarum —The Causative Agent of Bacterial Kidney Disease in Salmonid Fish. Pathogens 2020 , 9 , 845. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Elliott, D.G.; Wiens, G.D.; Hammell, K.L.; Rhodes, L.D. Vaccination against Bacterial Kidney Disease. Fish Vaccin. 2014 , 255–272. [ Google Scholar ] [ CrossRef ]
  • Brynildsrud, O.; Feil, E.J.; Bohlin, J.; Castillo-Ramirez, S.; Colquhoun, D.; McCarthy, U.; Matejusova, I.M.; Rhodes, L.D.; Wiens, G.D.; Verner-Jeffreys, D.W. Microevolution of Renibacterium salmoninarum : Evidence for intercontinental dissemination associated with fish movements. ISME J. 2014 , 8 , 746–756. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Matejusova, I.; Bain, N.; Colquhoun, D.J.; Feil, E.J.; McCarthy, U.; McLennan, D.; Snow, M.; Verner-Jeffreys, D.; Wallace, I.S.; Weir, S.J.; et al. Multilocus variable-number tandem-repeat genotyping of Renibacterium salmoninarum , a bacterium causing bacterial kidney disease in salmonid fish. BMC Microbiol. 2013 , 13 , 285. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bethke, J.; Poblete-Morales, M.; Irgang, R.; Yáñez, A.; Avendaño-Herrera, R. Iron acquisition and siderophore production in the fish pathogen Renibacterium salmoninarum . J. Fish Dis 2016 , 39 , 1275–1283. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zhou, T.; Yuan, Z.; Tan, S.; Jin, Y.; Yang, Y.; Shi, H.; Wang, W.; Niu, D.; Gao, L.; Jiang, W.; et al. A Review of Molecular Responses of Catfish to Bacterial Diseases and Abiotic Stresses. Front. Physiol. 2018 , 9 , 1113. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Mohammed, H.; Olivares-Fuster, O.; LaFrentz, S.; Arias, C.R. New attenuated vaccine against columnaris disease in fish: Choosing the right parental strain is critical for vaccine efficacy. Vaccine 2013 , 31 , 5276–5280. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Rathore, G. Bacterial Vaccines for Fishes: Current Status. Prophyl. Aquac. 2017 , 2 . Available online: https://krishi.icar.gov.in/jspui/bitstream/123456789/10377/1/Compendium%20on%20Prophylaxis%20in%20Aquaculture.pdf (accessed on 10 February 2024).
  • Bravo, S. Environmental Impacts and Management of Veterinary Medicines in Aquaculture: The Case of Salmon Aquaculture in Chile. Improving Biosecurity through Prudent and Responsible Use of Veterinary Medicines in Aquatic Food Production ; Food and Agriculture Organization of the United Nations: Rome, Italy, 2012; Volume 11. [ Google Scholar ]
  • Gavriilidou, A.; Gutleben, J.; Versluis, D.; Forgiarini, F.; van Passel, M.W.J.; Ingham, C.J.; Smidt, H.; Sipkema, D. Comparative genomic analysis of Flavobacteriaceae : Insights into carbohydrate metabolism, gliding motility and secondary metabolite biosynthesis. BMC Genom. 2020 , 21 , 569. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Dumetz, F.; Lapatra, S.E.; Duchaud, E.; Claverol, S.; Le Hénaff, M. The Flavobacterium psychrophilum OmpA, an outer membrane glycoprotein, induces a humoral response in rainbow trout. J. Appl. Microbiol. 2007 , 103 , 1461–1470. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bøgwald, J.; Dalmo, R.A. Review on Immersion Vaccines for Fish: An Update 2019. Microorganisms 2019 , 7 , 627. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Sudheesh, P.S.; Cain, K.D. Prospects and challenges of developing and commercializing immersion vaccines for aquaculture. Int. Biol. Rev. 2017 , 1 . [ Google Scholar ]
  • Ma, J.; Bruce, T.J.; Sudheesh, P.S.; Knupp, C.; Loch, T.P.; Faisal, M.; Cain, K.D. Assessment of cross-protection to heterologous strains of Flavobacterium psychrophilum following vaccination with a live-attenuated coldwater disease immersion vaccine. J. Fish Dis. 2019 , 42 , 75–84. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bruce, T.J.; Ma, J.; Knupp, C.; Loch, T.P.; Faisal, M.; Cain, K.D. Cross-protection of a live-attenuated Flavobacterium psychrophilum immersion vaccine against novel Flavobacterium spp. and Chryseobacterium spp. Strains. J. Fish Dis. 2020 , 43 , 915–928. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Fast, M.D.; Tse, B.; Boyd, J.M.; Johnson, S.C. Mutations in the Aeromonas salmonicida subsp. salmonicida type III secretion system affect Atlantic salmon leucocyte activation and downstream immune responses. Fish Shellfish Immunol. 2009 , 27 , 721–728. [ Google Scholar ] [ PubMed ]
  • Gudmundsdottir, B.K.; Bjornsdottir, B. Aeromonas salmonicida and A. hydrophila . In Fish Viruses and Bacteria: Pathobiology and Protection ; CABI: Boston, MA, USA, 2017. [ Google Scholar ]
  • Braden, L.M.; Whyte, S.K.; Brown, A.B.J.; Van Iderstine, C.; Letendre, C.; Groman, D.; Lewis, J.; Purcell, S.L.; Hori, T.; Fast, M.D. Vaccine-Induced Protection Against Furunculosis Involves Pre-emptive Priming of Humoral Immunity in Arctic Charr. Front. Immunol. 2019 , 10 , 120. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bergh, P.V.; Heller, M.; Braga-Lagache, S.; Frey, J. The Aeromonas salmonicida subsp. salmonicida exoproteome: Determination of the complete repertoire of Type-Three Secretion System effectors and identification of other virulence factors. Proteome Sci. 2013 , 11 , 42. [ Google Scholar ]
  • Mikkelsen, H.; Schrøder, M.B.; Lund, V. Vibriosis and atypical furunculosis vaccines; efficacy, specificity and side effects in Atlantic cod, Gadus morhua L. Aquaculture 2004 , 242 , 81–91. [ Google Scholar ] [ CrossRef ]
  • Rørstad, G.; Aasjord, P.M.; Robertsen, B. Adjuvant effect of a yeast glucan in vaccines against furunculosis in Atlantic salmon ( Salmo salar L.). Fish Shellfish. Immunol. 1993 , 3 , 179–190. [ Google Scholar ]
  • Toranzo, A.; Romalde, J.; Magariños, B.; Barja, J. Present and future of aquaculture vaccines against fish bacterial diseases. Options Mediterr. 2009 , 86 , 155–176. [ Google Scholar ]
  • Erkinharju, T.; Lundberg, M.R.; Isdal, E.; Hordvik, I.; Dalmo, R.A.; Seternes, T. Studies on the antibody response and side effects after intramuscular and intraperitoneal injection of Atlantic lumpfish ( Cyclopterus lumpus L.) with different oil-based vaccines. J. Fish Dis. 2017 , 40 , 1805–1813. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Marana, M.H.; Sepúlveda, D.; Chen, D.; Al-Jubury, A.; Jaafar, R.M.; Kania, P.W.; Henriksen, N.H.; Krossøy, B.; Dalsgaard, I.; Lorenzen, N.; et al. A pentavalent vaccine for rainbow trout in Danish aquaculture. Fish Shellfish Immunol. 2019 , 88 , 344–351. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Villumsen, K.R.; Kania, P.W.; Christensen, D.; Koppang, E.O.; Bojesen, A.M. Injection Vaccines Formulated with Nucleotide, Liposomal or Mineral Oil Adjuvants Induce Distinct Differences in Immunogenicity in Rainbow Trout. Vaccines 2020 , 8 , 103. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Hoare, R.; Jung, S.J.; Ngo, T.P.H.; Bartie, K.; Bailey, J.; Thompson, K.D.; Adams, A. Efficacy and safety of a non-mineral oil adjuvanted injectable vaccine for the protection of Atlantic salmon ( Salmo salar L.) against Flavobacterium psychrophilum . Fish Shellfish Immunol. 2019 , 85 , 44–51. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Tziouvas, H.; Varvarigos, A.P. Intensity scale of side effects in European sea bass ( Dicentrarchus labrax ) post intraperitoneal injection with commercial oil-adjuvanted vaccines. Bull. Eur. Assoc. Fish Pathol. 2021 , 41 , 8. [ Google Scholar ] [ CrossRef ]
  • Thornton, J.C.; Garduno, R.A.; Kay, W.W. The development of live vaccines for furunculosis lacking the A-layer and O-antigen of Aeromonas salmonicida . J. Fish Dis. 1994 , 17 , 195–204. [ Google Scholar ] [ CrossRef ]
  • Menanteau-Ledouble, S.; El-Matbouli, M. Antigens of Aeromonas salmonicida subsp. salmonicida specifically induced in vivo in Oncorhynchus mykiss . J. Fish Dis. 2016 , 39 , 1015–1019. [ Google Scholar ] [ PubMed ]
  • Ling, X.-D.; Dong, W.; Zhang, Y.; Hu, J.; Liu, J.-X.; Zhao, X. A recombinant adenovirus targeting typical Aeromonas salmonicida induces an antibody-mediated adaptive immune response after immunization of rainbow trout. Microb. Pathog. 2019 , 133 , 103559. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Van Doan, H.; Soltani, M.; Leitão, A.; Shafiei, S.; Asadi, S.; Lymbery, A.J.; Ringø, E. Streptococcosis a Re-Emerging Disease in Aquaculture: Significance and Phytotherapy. Animals 2022 , 12 , 2443. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zlotkin, A.; Chilmonczyk, S.; Eyngor, M.; Hurvitz, A.; Ghittino, C.; Eldar, A. Trojan horse effect: Phagocyte-mediated Streptococcus iniae infection of fish. Infect. Immunity 2003 , 71 , 2318–2325. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Shoemaker, C.; DeHai, X.D.X.; Soto, E. Streptococcus iniae and S. agalactiae ; CABI: Wallingford, UK, 2017; pp. 298–313. [ Google Scholar ]
  • Liu, Y.; Li, L.; Yu, F.; Luo, Y.; Liang, W.; Yang, Q.; Wang, R.; Li, M.; Tang, J.; Gu, Q.; et al. Genome-wide analysis revealed the virulence attenuation mechanism of the fish-derived oral attenuated Streptococcus iniae vaccine strain YM011. Fish Shellfish Immunol. 2020 , 106 , 546–554. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zhang, D.; Gao, Y.; Li, Q.; Ke, X.; Liu, Z.; Lu, M.; Shi, C. An effective live attenuated vaccine against Streptococcus agalactiae infection in farmed Nile tilapia ( Oreochromis niloticus ). Fish Shellfish Immunol. 2020 , 98 , 853–859. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Huang, L.Y.; Wang, K.Y.; Xiao, D.; Chen, D.F.; Geng, Y.; Wang, J.; He, Y.; Wang, E.L.; Huang, J.L.; Xiao, G.Y. Safety and immunogenicity of an oral DNA vaccine encoding Sip of Streptococcus agalactiae from Nile tilapia Oreochromis niloticus delivered by live attenuated Salmonella typhimurium . Fish Shellfish Immunol. 2014 , 38 , 34–41. [ Google Scholar ] [ CrossRef ]
  • Munang, H.M.; Paul, J.; Evensen, Ø. An Overview of Vaccination Strategies and Antigen Delivery Systems for Streptococcus agalactiae Vaccines in Nile Tilapia ( Oreochromis niloticus ). Vaccines 2016 , 4 , 48. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Heckman, T.I.; Shahin, K.; Henderson, E.E.; Griffin, M.J.; Soto, E. Development and efficacy of Streptococcus iniae live-attenuated vaccines in Nile tilapia, Oreochromis niloticus . Fish Shellfish Immunol. 2022 , 121 , 152–162. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Luo, Z.-W.; Jiang, Y.-H.; Lin, L.-B.; Deng, X.-Y.; Zhang, Q.; Zhang, Q.-L. Genome-wide differential expression analysis explores antibacterial molecular mechanisms of zebrafish intestine upon pathogenic Streptococcus agalactiae challenge. Aquac. Rep. 2021 , 19 , 100639. [ Google Scholar ] [ CrossRef ]
  • Kumar, G.; Menanteau-Ledouble, S.; Saleh, M.; El-Matbouli, M. Yersinia ruckeri , the causative agent of enteric redmouth disease in fish. Vet. Res. 2015 , 46 , 103. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Çagirgan, H.; Tanrikul, T. Testing the effectiveness of a Yersinia vaccine in infected and chemically treated juvenile rainbow trout ( Oncorhynchus mykiss ). J. Appl. Ichthyol. 1998 , 14 , 239–243. [ Google Scholar ] [ CrossRef ]
  • Villumsen, K.R.; Neumann, L.; Ohtani, M.; Strøm, H.K.; Raida, M.K. Oral and anal vaccination confers full protection against enteric redmouth disease (ERM) in rainbow trout. PLoS ONE 2014 , 9 , e93845. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Jaafar, R.M.; Al-Jubury, A.; Dalsgaard, I.; MohammadKarami, A.; Kania, P.W.; Buchmann, K. Effect of oral booster vaccination of rainbow trout against Yersinia ruckeri depends on type of primary immunization. Fish Shellfish Immunol. 2019 , 85 , 61–65. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Deshmukh, S.; Raida, M.K.; Dalsgaard, I.; Chettri, J.K.; Kania, P.W.; Buchmann, K. Comparative protection of two different commercial vaccines against Yersinia ruckeri serotype O1 and biotype 2 in rainbow trout ( Oncorhynchus mykiss ). Vet. Immunol. Immunopathol. 2012 , 145 , 379–385. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zorriehzahra, M.J.; Adel, M.; Delshad, S.T. Enteric redmouth disease: Past, present, future: A review. Iran. J. Fish. Sci. 2017 , 16 , 1135–1156. [ Google Scholar ]
  • Darvishi, M.; Mehrgan, M.S.; Khajehrahimi, A.E. Effect of Licorice ( Glycyrrhiza glabra ) Extract as an Immunostimulant on Serum and Skin Mucus Immune Parameters, Transcriptomic Responses of Immune-Related Gene, and Disease Resistance Against Yersinia ruckeri in Rainbow Trout ( Oncorhynchus mykiss ). Front. Vet. Sci. 2022 , 9 , 811684. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Parrino, V.; Kesbiç, O.S.; Acar, Ü.; Fazio, F. Hot pepper ( Capsicum sp.) oil and its effects on growth performance and blood parameters in rainbow trout ( Oncorhynchus mykiss ). Nat. Prod. Res. 2019 , 34 , 3226–3230. [ Google Scholar ] [ CrossRef ]
  • Morita, H.; Toh, H.; Oshima, K.; Yoshizaki, M.; Kawanishi, M.; Nakaya, K.; Suzuki, T.; Miyauchi, E.; Ishii, Y.; Tanabe, S.; et al. Complete Genome Sequence and Comparative Analysis of the Fish Pathogen Lactococcus garvieae . PLoS ONE 2011 , 6 , e23184. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Eldar, A.A.; Ghittino, C. Lactococcus garvieae and Streptococcus iniae infections in rainbow trout Oncorhynchus mykiss : Similar, but different diseases. Dis. Aquat. Org. 1999 , 36 , 227–231. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Shahi, N.; Mallik, S.K. Emerging bacterial fish pathogen Lactococcus garvieae RTCLI04, isolated from rainbow trout ( Oncorhynchus mykiss ): Genomic features and comparative genomics. Microb. Pathog. 2020 , 147 , 104368. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Khalil, S.M.I.; Bulfon, C.; Galeotti, M.; Acutis, P.L.; Altinok, I.; Kotzamanidis, C.; Vela, A.I.; Fariano, L.; Prearo, M.; Colussi, S.; et al. Immune profiling of rainbow trout ( Oncorhynchus mykiss ) exposed to Lactococcus garvieae : Evidence in asymptomatic versus symptomatic or vaccinated fish. J. Fish Dis. 2023 , 46 , 731–741. [ Google Scholar ] [ CrossRef ]
  • Bwalya, P.; Hang, B.M.; Gamil, A.A.; Munang, H.M.; Evensen, Ø.; Mutoloki, S. A whole-cell Lactococcus garvieae autovaccine protects Nile tilapia against infection. PLoS ONE 2020 , 15 , e0230739. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Fukushima, H.C.S.; Leal, C.A.G.; Cavalcante, R.B.; Figueiredo, H.C.P.; Arijo, S.; Moriñigo, M.A.; Ishikawa, M.; Borra, R.C.; Ranzani-Paiva, M.J.T. Lactococcus garvieae outbreaks in Brazilian farms Lactococcosis in Pseudoplatystoma sp.—Development of an autogenous vaccine as a control strategy. J. Fish Dis. 2017 , 40 , 263–272. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Halimi, M.; Alishahi, M.; Abbaspour, M.R.; Ghorbanpoor, M.; Tabandeh, M.R. High efficacy and economical procedure of oral vaccination against Lactococcus garvieae / Streptococcus iniae in rainbow trout ( Oncorhynchus mykiss ). Fish Shellfish Immunol. 2020 , 99 , 505–513. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Halimi, M.; Alishahi, M.; Abbaspour, M.R.; Ghorbanpoor, M.; Tabandeh, M.R. Valuable method for production of oral vaccine by using alginate and chitosan against Lactococcus garvieae / Streptococcus iniae in rainbow trout ( Oncorhynchus mykiss ). Fish Shellfish Immunol. 2019 , 90 , 431–439. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Su, F.J.; Chen, M.M. Protective Efficacy of Novel Oral Biofilm Vaccines against Lactococcus garvieae Infection in Mullet, Mugil cephalus. Vaccines 2021 , 9 , 844. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Rathore, G.; Kumar, G.; Swaminathan, T.R.; Swain, P. Koi herpes virus: A review and risk assessment of Indian aquaculture. Indian J. Virol. Off. Organ Indian Virol. Soc. 2012 , 23 , 124–133. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Bergmann, S.M.; Jin, Y.; Franzke, K.; Grunow, B.; Wang, Q.; Klafack, S. Koi herpesvirus (KHV) and KHV disease (KHVD)—A recently updated overview. J. Appl. Microbiol. 2020 , 129 , 98–103. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Schröder, L.; Klafack, S.; Bergmann, S.M.; Fichtner, D.; Jin, Y.; Lee, P.Y.; Höper, D.; Mettenleiter, T.C.; Fuchs, W. Generation of a potential koi herpesvirus live vaccine by simultaneous deletion of the viral thymidine kinase and dUTPase genes. J. Gen. Virol. 2019 , 100 , 642–655. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Cui, L.-C.; Guan, X.-T.; Liu, Z.-M.; Tian, C.-Y.; Xu, Y.-G. Recombinant lactobacillus expressing G protein of spring viremia of carp virus (SVCV) combined with ORF81 protein of koi herpesvirus (KHV): A promising way to induce protective immunity against SVCV and KHV infection in cyprinid fish via oral vaccination. Vaccine 2015 , 33 , 3092–3099. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Hu, F.; Li, Y.; Wang, Q.; Wang, G.; Zhu, B.; Wang, Y.; Zeng, W.; Yin, J.; Liu, C.; Bergmann, S.M.; et al. Carbon nanotube-based DNA vaccine against koi herpesvirus given by intramuscular injection. Fish Shellfish Immunol. 2020 , 98 , 810–818. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Peñaranda, M.M.D.; LaPatra, S.E.; Kurath, G. Specificity of DNA vaccines against the U and M genogroups of infectious hematopoietic necrosis virus (IHNV) in rainbow trout ( Oncorhynchus mykiss ). Fish Shellfish Immunol. 2011 , 31 , 43–51. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Larragoite, E.T.; Tacchi, L.; LaPatra, S.E.; Salinas, I. An attenuated virus vaccine appears safe to the central nervous system of rainbow trout ( Oncorhynchus mykiss ) after intranasal delivery. Fish Shellfish Immunol. 2016 , 49 , 351–354. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Tang, L.; Kang, H.; Duan, K.; Guo, M.; Lian, G.; Wu, Y.; Li, Y.; Gao, S.; Jiang, Y.; Yin, J.; et al. Effects of Three Types of Inactivation Agents on the Antibody Response and Immune Protection of Inactivated IHNV Vaccine in Rainbow Trout. Viral Immunol. 2016 , 29 , 430–435. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Rouxel, R.N.; Tafalla, C.; Mérour, E.; Leal, E.; Biacchesi, S.; Brémont, M.; Lyles, D.S. Attenuated Infectious Hematopoietic Necrosis Virus with Rearranged Gene Order as Potential Vaccine. J. Virol. 2016 , 90 , 10857–10866. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Long, A.; Richard, J.; Hawley, L.; LaPatra, S.E.; Garver, K.A. Transmission potential of infectious hematopoietic necrosis virus in APEX-IHN®-vaccinated Atlantic salmon. Dis. Aquat. Organ. 2017 , 122 , 213–221. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Yong, C.Y.; Ong, H.K.; Tang, H.C.; Yeap, S.K.; Omar, A.R.; Ho, K.L.; Tan, W.S. Infectious hematopoietic necrosis virus: Advances in diagnosis and vaccine development. PeerJ 2019 , 7 , e7151. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Caipang, C.M.; Takano, T.; Hirono, I.; Aoki, T. Genetic vaccines protect red seabream, Pagrus major , upon challenge with red seabream iridovirus (RSIV). Fish Shellfish Immunol. 2006 , 21 , 130–138. [ Google Scholar ] [ CrossRef ]
  • Nakajima, K.; Maeno, Y.; Honda, A.; Yokoyama, K.; Tooriyama, T.; Manabe, S. Effectiveness of a vaccine against red sea bream iridoviral disease in a field trial test. Dis. Aquat. Organ. 1999 , 36 , 73–75. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Jung, M.H.; Nikapitiya, C.; Jung, S.J. DNA vaccine encoding myristoylated membrane protein (MMP) of rock bream iridovirus (RBIV) induces protective immunity in rock bream ( Oplegnathus fasciatus ). Vaccine 2018 , 36 , 802–810. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Oh, S.Y.; Oh, M.J.; Nishizawa, T. Potential for a live red seabream iridovirus (RSIV) vaccine in rock bream Oplegnathus fasciatus at a low rearing temperature. Vaccine 2014 , 32 , 363–368. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Senapin, S.; Dong, H.T.; Meemetta, W.; Gangnonngiw, W.; Sangsuriya, P.; Vanichviriyakit, R.; Sonthi, M.; Nuangsaeng, B. Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia. J. Fish Dis. 2019 , 42 , 119–127. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Thanasaksiri, K.; Fukuda, K.; Tsubone, S.; Miyadai, H.; Murakami, T.; Murakami, A.; Takano, R. Efficacy of a bivalent inactivated vaccine against red seabream iridovirus and Streptococcus iniae in red seabream, Pagrus major . Aquaculture 2018 , 492 , 132–136. [ Google Scholar ]
  • Deperasińska, I.; Schulz, P.; Siwicki, A.K.; Alphavirus, S. Salmonid alphavirus (SAV). J. Vet. Res. 2018 , 62 , 1–6. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Graham, D.A.; Rowley, H.R.; Frost, P. Cross-neutralization studies with salmonid alphavirus subtype 1–6 strains: Results with sera from experimental studies and natural infections. J. Fish Dis. 2014 , 37 , 683–691. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Jensen, B.B.; Kristoffersen, A.B.; Myr, C.; Brun, E. Cohort study of effect of vaccination on pancreas disease in Norwegian salmon aquaculture. Dis. Aquat. Organ. 2012 , 102 , 23–31. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Chang, C.J.; Gu, J.; Robertsen, B. Protective effect and antibody response of DNA vaccine against salmonid alphavirus 3 (SAV3) in Atlantic salmon. J. Fish Dis. 2017 , 40 , 1775–1781. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Collins, C.; Lester, K.; Del-Pozo, J.; Collet, B. Non-Lethal Sequential Individual Monitoring of Viremia in Relation to DNA Vaccination in Fish-Example Using a Salmon Alphavirus DNA Vaccine in Atlantic Salmon Salmo salar . Vaccines 2021 , 9 , 163. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Pajdak-Czaus, J.; Schulz, P.; Terech-Majewska, E.; Szweda, W.; Siwicki, A.K.; Platt-Samoraj, A. Influence of Infectious Pancreatic Necrosis Virus and Yersinia ruckeri Co-Infection on a Non-Specific Immune System in Rainbow Trout ( Oncorhynchus mykiss ). Animals 2021 , 11 , 1974. [ Google Scholar ] [ CrossRef ]
  • Kanrar, S.; Dhar, A.K. Complete Genome Sequence of a Novel Mutant Strain of Vibrio parahaemolyticus from Pacific White Shrimp ( Penaeus vannamei ). Genome Announc. 2018 , 6 , e00497-18. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Ballesteros, N.A.; Saint-Jean, S.R.; Perez-Prieto, S.I. Food pellets as an effective delivery method for a DNA vaccine against infectious pancreatic necrosis virus in rainbow trout ( Oncorhynchus mykiss , Walbaum). Fish Shellfish Immunol. 2014 , 37 , 220–228. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Ahmadivand, S.; Soltani, M.; Behdani, M.; Evensen, Ø.; Alirahimi, E.; Soltani, E.; Hassanzadeh, R.; Ashrafi-Helan, J. VP2 (PTA motif) encoding DNA vaccine confers protection against lethal challenge with infectious pancreatic necrosis virus (IPNV) in trout. Mol. Immunol. 2018 , 94 , 61–67. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Kibenge, F.S.; Munir, K.; Kibenge, M.J.; Joseph, T.; Moneke, E. Infectious salmon anemia virus: Causative agent, pathogenesis and immunity. Anim. Health Res. Rev. 2004 , 5 , 65–78. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Kibenge, F.S.; Kibenge, M.J.; McDougall, J. The development of infectious salmon anemia virus vaccines in Canada. In International Response to Infectious Salmon Anemia: Prevention, Control, and Eradication: Proceedings of a Symposium, New Orleans, LA, USA, 3–4 September 2002 ; Miller, O., Cipriano, R.C., Eds.; Animal and Plant Health Inspection Service; US Department of the Interior, US Geological Survey; US Department of Commerce, National Marine Fisheries Service, New Orleans, LA. Technical Bulletin 1902; US Department of Agriculture: Washington, DC, USA, 2003; pp. 39–49. [ Google Scholar ]
  • Wolf, A.; Hodneland, K.; Frost, P.; Braaen, S.; Rimstad, E. A hemagglutinin-esterase-expressing salmonid alphavirus replicon protects Atlantic salmon ( Salmo salar ) against infectious salmon anemia (ISA). Vaccine 2013 , 31 , 661–669. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Chang, C.J.; Sun, B.; Robertsen, B. Adjuvant activity of fish type I interferon shown in a virus DNA vaccination model. Vaccine 2015 , 33 , 2442–2448. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Ahasan, M.S.; Keleher, W.; Giray, C.; Perry, B.; Surachetpong, W.; Nicholson, P.; Al-Hussinee, L.; Subramaniam, K.; Waltzek, T.B. Genomic Characterization of Tilapia Lake Virus Isolates Recovered from Moribund Nile Tilapia ( Oreochromis niloticus ) on a Farm in the United States. Microbiol. Resour. Announc. 2020 , 9 , e01368-19. [ Google Scholar ] [ PubMed ]
  • Abbadi, M.; Basso, A.; Biasini, L.; Quartesan, R.; Buratin, A.; Davidovich, N.; Toffan, A. Tilapia lake virus: A structured phylogenetic approach. Front. Genet. 2023 , 14 , 1069300. [ Google Scholar ] [ CrossRef ]
  • Jansen, M.D.; Dong, H.T.; Mohan, C.V. Tilapia lake virus: A threat to the global tilapia industry? Rev. Aquac. 2019 , 11 , 725–739. [ Google Scholar ] [ CrossRef ]
  • Thawornwattana, Y.; Dong, H.T.; Phiwsaiya, K.; Sangsuriya, P.; Senapin, S.; Aiewsakun, P. Tilapia lake virus (TiLV): Genomic epidemiology and its early origin. Transbound. Emerg. Dis. 2021 , 68 , 435–444. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Zeng, W.; Wang, Y.; Hu, H.; Wang, Q.; Bergmann, S.M.; Wang, Y.; Li, B.; Lv, Y.; Li, H.; Yin, J.; et al. Cell Culture-Derived Tilapia Lake Virus-Inactivated Vaccine Containing Montanide Adjuvant Provides High Protection against Viral Challenge for Tilapia. Vaccines 2021 , 9 , 86. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Mai, T.T.; Kayansamruaj, P.; Soontara, C.; Kerddee, P.; Nguyen, D.-H.; Senapin, S.; Costa, J.Z.; del-Pozo, J.; Thompson, K.D.; Rodkhum, C.; et al. Immunization of Nile Tilapia ( Oreochromis niloticus ) Broodstock with Tilapia Lake Virus (TiLV) Inactivated Vaccines Elicits Protective Antibody and Passive Maternal Antibody Transfer. Vaccines 2022 , 10 , 167. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Shivam, S.; El-Matbouli, M.; Kumar, G. Development of Fish Parasite Vaccines in the OMICs Era: Progress and Opportunities. Vaccines 2021 , 9 , 179. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Barrett, L.T.; Oppedal, F.; Robinson, N.; Dempster, T. Prevention not cure: A review of methods to avoid sea lice infestations in salmon aquaculture. Rev. Aquac. 2020 , 12 , 2527–2543. [ Google Scholar ] [ CrossRef ]
  • Sitjà-Bobadilla, A. Living off a fish: A trade-off between parasites and the immune system. Fish Shellfish Immunol. 2008 , 25 , 358–372. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Khan, R.A. Host-Parasite Interactions in Some Fish Species. J. Parasitol. Res. 2012 , 2012 , 237280. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Sfacteria, A.; Brines, M.; Blank, U. The mast cell plays a central role in the immune system of teleost fish. Mol. Immunol. 2015 , 63 , 3–8. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Dezfuli, B.S.; Bo, T.; Lorenzoni, M.; Shinn, A.P.; Giari, L. Fine structure and cellular responses at the host-parasite interface in a range of fish-helminth systems. Vet. Parasitol. 2015 , 208 , 272–279. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Faber, M.N.; Holland, J.W.; Secombes, C.J. Vaccination strategies and IgM responses against PKD in rainbow trout. Fish Shellfish Immunol. 2019 , 91 , 423. [ Google Scholar ] [ CrossRef ]
  • Carpio, Y.; Basabe, L.; Acosta, J.; Rodríguez, A.; Mendoza, A.; Lisperger, A.; Zamorano, E.; González, M.; Rivas, M.; Contreras, S.; et al. Novel gene isolated from Caligus rogercresseyi: A promising target for vaccine development against sea lice. Vaccine 2011 , 29 , 2810–2820. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Guragain, P.; Tkachov, M.; Båtnes, A.S.; Olsen, Y.; Winge, P.; Bones, A.M. Principles and Methods of Counteracting Harmful Salmon–Arthropod Interactions in Salmon Farming: Addressing Possibilities, Limitations, and Future Options. Front. Mar. Sci. 2021 , 8 , 701793. [ Google Scholar ] [ CrossRef ]
  • Yao, J.Y.; Yuan, X.M.; Xu, Y.; Yin, W.L.; Lin, L.Y.; Pan, X.Y.; Yang, G.L.; Wang, C.F.; Shen, J.Y. Live recombinant Lactococcus lactis vaccine expressing immobilization antigen (i-Ag) for protection against Ichthyophthirius multifiliis in goldfish. Fish Shellfish Immunol. 2016 , 58 , 302–308. [ Google Scholar ] [ CrossRef ]
  • Jørgensen, L.V.G. The fish parasite Ichthyophthirius multifiliis —Host immunology, vaccines and novel treatments. Fish Shellfish Immunol. 2017 , 67 , 586–595. [ Google Scholar ] [ CrossRef ]
  • von Gersdorff Jørgensen, L.; Kania, P.W.; Rasmussen, K.J.; Mattsson, A.H.; Schmidt, J.; Al-Jubury, A.; Sander, A.; Salanti, A.; Buchmann, K. Rainbow trout ( Oncorhynchus mykiss ) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis . J. Fish Dis. 2017 , 40 , 1815–1821. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Kang, M.; Feng, F.; Wang, Y.; Guo, L.; Chen, L.; Chen, K. Advances in Research into Oral Vaccines for Fish. Int. J. Fish. Aquac. Sci. 2018 , 8 , 19–40. [ Google Scholar ]
  • Embregts, C.W.E.; Forlenza, M. Oral vaccination of fish: Lessons from humans and veterinary species. Dev. Comp. Immunol. 2016 , 64 , 118–137. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Munang, H.M.; Mutoloki, S.; Evensen, Ø. An Overview of Challenges Limiting the Design of Protective Mucosal Vaccines for Finfish. Front. Immunol. 2015 , 6 , 542. [ Google Scholar ]
  • Neutra, M.R.; Kozlowski, P.A. Mucosal vaccines: The promise and the challenge. Nat. Rev. Immunol. 2006 , 6 , 148–158. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Cao, Z.; Liu, S.; Nan, H.; Zhao, K.; Xu, X.; Wang, G.; Ji, H.; Chen, H. Immersion immunization with recombinant baculoviruses displaying cyprinid herpesvirus 2 membrane proteins induced protective immunity in gibel carp. Fish Shellfish Immunol. 2019 , 93 , 879–887. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Li, S.; Xie, H.; Yan, Z.; Li, B.; Wu, P.; Qian, X.; Zhang, X.; Wu, J.; Liu, J.; Zhao, X. Development of a live vector vaccine against infectious hematopoietic necrosis virus in rainbow trout. Fish Shellfish. Immunol. 2019 , 89 , 516–524. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Clarke, J.L.; Waheed, M.T.; Lössl, A.G.; Martinussen, I.; Daniell, H. How can plant genetic engineering contribute to cost-effective fish vaccine development for promoting sustainable aquaculture? Plant Mol. Biol. 2013 , 83 , 33–40. [ Google Scholar ] [ CrossRef ] [ PubMed ]
  • Yimer, M.; Tesfaye, S.; Birhanu, B. Present Status and Future Prospects of Fish Vaccination: A Review. J. Vet. Sci. Technol. 2016 , 7 , 1000299. [ Google Scholar ]
  • Flores-Kossack, C.; Montero, R.; Köllner, B.; Maisey, K. Chilean aquaculture and the new challenges: Pathogens, immune response, vaccination and fish diversification. Fish Shellfish Immunol. 2020 , 98 , 52–67. [ Google Scholar ] [ CrossRef ] [ PubMed ]

Click here to enlarge figure

Name of the DiseasesCausative AgentFish It Affects
Bacterial
Atypical furunculosisAeromonas salmonicidaSalmonids, spotted wolfish, Atlantic cod
Motile aeromonid septicemiaAeromonas hydrophila, A. caviae, A. veronii biovar sobriaFreshwater fish species, including catfish and brass
VibriosisVibrio spp., including V. harveyi, V. vulnificus, V. alginolyticus, and V. parahaemolyticusMarine fish including salmonids, yellowtail, halibut, amberjack
Enteric septicemiaEdwardsiella ictaluriCatfish
EdwardsiellosisEdwardsiella tardaCatfish, striped bass, tilapia, sea bream
TuberculosisMycobacterium marinum, M. fortuitum, M. chelonaeMarine, brackish, and freshwater fish, including sea bass, tropical aquarium fish
Rainbow trout fry syndrome/Bacterial Cold-Water DiseaseFlavobacterium psychrophilumSalmonids, freshwater fish
ColumnarisFlavobacterium columnareCyprinids, trout, tilapia
Streptococcosis Streptococcus agalactiae Tilapia, bass, rainbow trout
StreptococcosisStreptococcus parauberisOlive flounder, rainbow trout, tilapia, bass
StreptococcosisStreptococcusiniaeAtlantic salmon, rainbow trout, and tilapia
Enteric redmouth disease/YersiniosisYersinia ruckeriSalmonids, rainbow trout, eel, minnows, sturgeon, and crustaceans
LactococcosisLactococcus garvieaeRainbow trout, yellowtail, catfish, olive flounder, greytail mullet, amberjack, kingfish
Viral
Tilapia Lake VirusTilapia TilapinevirusTilapia and hybrid tilapia fish
Infectious Hemorrhagic necrosis virusNovirhabdovirusTrout and salmon
Infectious salmon anemiaOrthomyxovirusAtlantic salmon, rainbow trout, coho salmon
Infectious pancreatic necrosisBirnavirusSalmonids, sea brass, sea bream, Pacific cod
Koi Herpes VirusHerpesvirusCyprinus carpio
Red Sea Bream IridovirusIridovirusMarine fish species including red sea bream, japanese seabass, and striped jack
Salmonid Alphavirus AlphavirusAtlantic salmon, rainbow trout
Iridoviral diseaseIridovirusAmberjack, yellowtail, red sea bream
Parasites
CostiasisIchthyobodo necotorSeveral freshwater and saltwater fish
Salmon Poisoning diseaseNanophyetus salmincolaSalmon, several freshwater fish
White SpotIchthyophthirius mulifiliisFreshwater fish
Sea LiceLepeophtheirus solmonisMarine salmonids
Whirling DiseaseMyxobolus cerebralisTrout, salmon, whitefish
MyxosporeansMyxobolus generaFreshwater and marine fish
MicrosporeanPleistophora generaFreshwater and marine fish
DiseasePathogenHostType of VaccineRoute of DeliveryTrade NameCountry
Enteric septicaemia of catfish (ESC)Edwardsiella ictaluricatfishLive attenuatedImmersionAquavac-ESCUS
Bacterial Kidney Disease (BKD)Renibacterium salmoninarumsalmonidsLive attenuatedIPRenogenUS
Canada
Chile
Flavobacteriosis/ColumnarisFlavobacterium columnare
Flavobacterium maritimus
cyprinids, salmonids, catfishLive attenuatedImmersionAquavac-ColUS
Canada
Chile
InactivatedIPAlpha Ject IPNVFlevo 0.025Chile
Killed bacterinImmersionFryVacc 1US
Canada
FryVacc 2Chile
FurunculosisAeromonas salmonicidaAtlantic salmon and rainbow troutInactivated, oil-basedIPAlphaJect 3000Denmark
Finland
Iceland
Ireland
Norway
Sweden
Alpha Ject 2.2UK
Alpha Ject 4-1, Alpha Ject 5-1Chile
Alpha Ject 6-2Norway
The Faroe Islands
Alpha Ject micro 7 ILANorway
The Faroe Islands
Subunit vaccineIPNorvax Minova 6Norway
Inactivated bacterin IPAquaVac-FNMUK
Ireland
Spain
France
Killed bacterinIPLipogen Forte, Furogen Dip, Forte VIUS
Canada
Streptococcosis Streptococcus iniaetilapia and seabassInactivatedIP or BathNorvax Strep Si, Aquavac Strep SaVietnam
Honduras
Indonesia
tilapiaKilled IPAquavac-GarvetilHonduras Venezuela
Ecuador The Philippines Indonesia
Streptococcus agalactiaetilapiaInactivatedIP AlphaJect micro1 TiLaBrazil
Colombia
Honduras
Indonesia
Panama
StreptococcusparauberisturbotInactivatedIPIcthiovac-STRSpain
VibriosisV. anguillarum
V. ordalii
Atlantic salmonInactivated, oil-basedIPAlpha Ject micro 7 ILA, Alpha Ject 6-2Norway
The Faroe Islands
Inactivated, oil-basedIPAlpha Ject 5-1, Alpha Ject 4-1,Chile
Inactivated, oil-basedIPAlpha Ject Micro-4Canada
Subunit vaccineIPNorvax Minova 6Norway
Inactivated, oil-basedIPAlpha Ject micro 6Ireland
UK
The Faroe Islands
Norway
sea bassInactivated, oil-basedIPAlpha Ject micro 2000Croatia
Spain
Greece
France
Atlantic salmonInactivated, oil-basedIPAlpha Ject Micro-3Chile
Atlantic salmon and
rainbow trout
Inactivated, oil-basedIPAlpha Ject 5-3Iceland
Norway
Atlantic salmon and
rainbow trout
Inactivated, oil-basedIPAlphaJect 3000Denmark
Finland
Iceland
Ireland
Norway
Sweden
Sea bassInactivated, oil-basedDipALPHA DIP VibCroatia
Cyprus
Greece
Italy
Portugal
Spain
Sea bassInactivated, oil-basedBath/
Immersion
ALPHA DIP VibrioTurkey
Atlantic salmonInactivated, oil-basedIPAlpha Ject 2-2UK
salmonidsKilled bacterinIPFurogen Dip,
Forte VI,
Lipogen Forte
US
Canada
salmonidsKilled bacterinBath/
Immersion
Vibrogen-2US
Canada
European sea bassInactivated bacterinIPAquaVac Vibrio PasteurellaGreece
Middle East
rainbow troutInactivated bacterinOral/
Immersion
AquaVac Vibrio, AquaVac Vibrio Oral BoostFinland
UK
Ireland
Spain
Greece
VirusType of Virus (RNA/DNA)Fish HostTrade Name (If Applicable)Type of VaccineDelivery MethodLicensed for Use in the Following CountriesDescription
SAVRNAAtlantic salmonNorvax Compact PDInactivatedIntraperitoneal InjectionNorway
Chile
UK
A monovalent vaccine which contains an inactivated strain of SAV subtype 1.
SAVRNAAtlantic salmonAquavac PD7InactivatedIntraperitoneal InjectionNorwayA polyvalent vaccine which contains seven strains to protect against
pancreatic disease, infectious pancreatic necrosis, furunculosis, cold-water vibriosis, vibriosis and winter ulcers. Specifically, to protect against SAV, it contains an inactivated strain of SAV subtype 1.
SAVRNAAtlantic salmonAquavac PD3InactivatedIntraperitoneal InjectionUKA polyvalent vaccine which contains an inactivated strain of SAV subtype 1, as well as infectious pancreatic necrosis and furunculosis.
SAVRNAAtlantic salmonAlphaject Micro 1 PDInactivatedIntraperitoneal InjectionUK
Norway
A monovalent vaccine which contains the inactivated SAV subtype 3, the SAV strain most dominant in Norway.
IPNVRNAAtlantic salmon, rainbow troutAlphaJect 1000InactivatedIntraperitoneal InjectionChile Norway UKA monovalent vaccine containing an inactivated form of the virus.
IPNVRNAAtlantic salmonBirnagen ForteInactivatedIntraperitoneal InjectionCanada
UK
A monovalent vaccine containing inactivated bacterins and virulins.
IPNVRNAAtlantic salmonAquavac IPN OralRecombinantOralUS
Canada
Chile
Middle East
A monovalent vaccine containing capsid proteins VP2 and VP3.
IPNVRNAAtlantic salmon, Pacific salmon, chinook salmon, rainbow troutBlueguard IPNV OralInactivatedOralChileA monovalent vaccine containing two inactivated strains of IPNV.
IPNVRNARainbow trout,
Atlantic salmon,
Pacific Salmon,
chinook salmon
Blueguard IPN InyectableInactivatedIntraperitoneal InjectionChileA monovalent vaccine containing two strains of inactivated IPNV.
IPNVRNAAtlantic salmonAlphaJect IPNV-Flavo 0.025InactivatedIntraperitoneal InjectionChileA bivalent vaccine protecting against IPNV and Flavobacteriosis.
IPNVRNAAtlantic salmon, Pacific salmon, rainbow troutAlphaJect Micro 2InactivatedIntraperitoneal InjectionChileA bivalent vaccine protecting against IPNV and SRS.
IPNVRNAAtlantic salmonAlphaJect 2-2InactivatedIntraperitoneal InjectionUKA bivalent vaccine protecting against IPNV and Furunculosis.
IPNVRNAAtlantic salmonAlphaJect Micro 3InactivatedIntraperitoneal InjectionChileA trivalent vaccine protecting against IPNV, SRS, and Vibriosis.
IPNVRNAAtlantic salmon,
rainbow trout
blueguard SRS+IPN+VibrioInactivatedIntraperitoneal InjectionChileA trivalent vaccine which includes two strains of inactivated IPNV and inactivated bacterins to protect against SRS and Vibrio.
IPNVRNAAtlantic salmonAlphaJect 4-1InactivatedIntraperitoneal InjectionChileA polyvalent vaccine protecting against Furunculosis, SRS, Vibriosis, and IPNV.
IPNVRNAAtlantic salmonPentium Forte PlusInactivatedIntraperitoneal InjectionNorwayContains inactivated whole virus of IPNV, and also protects against Furunculosis, Classical Vibriosis, coldwater vibriosis, and Winter Ulcer.
IPNVRNAAtlantic SalmonNorvax Minova 6Subunit, inactivatedIntraperitoneal InjectionUK
Norway
A multivalent vaccine which protects against Furunculosis, classical vibriosis, coldwater vibriosis, wound disease and IPNV. It contains a subunit VP2 capsid protein.
IPNVRNAAtlantic salmonAlphaJect Micro 6InactivatedIntraperitoneal InjectionNorway
United Kingdom
The Faroe Islands
Ireland
A multivalent vaccine protecting against Furunculosis, Vibriosis,
cold-water vibriosis,
Winter sore, and IPNV.
IPNVRNAAtlantic SalmonAlphaJect 6-2InactivatedIntraperitoneal InjectionNorway
The Faroe Islands
A polyvalent vaccine protecting against Furunculosis, Vibriosis. Coldwater vibriosis, Winter sore, and IPNV.
IPNV and ISARNAAtlantic salmonAlphaJect Micro 4-2InactivatedIntraperitoneal InjectionChileA multivalent vaccine protecting against IPNV, Infectious Salmon Anemia (ISA), Vibriosis, and Furunculosis.
IPNV and ISARNAAtlantic salmonAlphaJect 5-1InactivatedIntraperitoneal InjectionChileA polyvalent vaccine protecting against Furunculosis, SRS, Vibriosis, ISA, and IPNV.
IPNV and ISARNAAtlantic salmonAlphaJect Micro 7InactivatedIntraperitoneal InjectionNorway
The Faroe Islands
A multivalent vaccine protecting against, Furunculosis, Vibriosis, Coldwater vibriosis, Winter sore, IPNV, and (ISA).
IPNV and ISARNAAtlantic salmonBlueguard SRS+IPN+VO+ISASubunit and InactivatedIntraperitoneal InjectionChileA polyvalent vaccine containing subunit ISA, inactivated IPNV strain, and bacterins. It protects against ISA, IPNV, SRS, and Vibriosis.
IPNV and ISARNAAtlantic salmonBlueguard IPN+SRS+AS+VO+ISA inyectableSubunit and InactivatedIntraperitoneal InjectionChileA polyvalent vaccine containing subunit ISA, inactivated IPNV strain, and bacterins. It protects against ISA, IPN, SRS, vibriosis, and furunculosis.
ISARNAAtlantic salmonAlphaJect Micro 1 ISAInactivatedIntraperitoneal InjectionChileA monovalent vaccine that includes an inactivated strain of ISA.
ISARNASalmonidsForte VIIInactivatedIntraperitoneal InjectionCanadaA polyvalent vaccine which contains inactivated ISA and bacterin. It protects against ISA, Furunculosis, and Vibriosis.
RSIVDNARed sea bream, yellowtail and sea brassn.aFormalinIntraperitonealJapanA monovalent formalin-based vaccine that fights against RSIV. This was the first vaccine made against the virus.
RSIVDNARed sea bream, yellowtail and sea brassAQUAVAC IridoVFormalin, oil-adjuvantIntraperitonealSingaporeA monovalent vaccine with an inactivated strain of RSIV which targets tilapia and Asian sea bass.
IHNVRNASalmonids including rainbow trout, steelhead trout and Atlantic salmonApex-IHN DNAIntramuscular InjectionCanada, USAA DNA plasmid vaccine targeting IHNV in salmonids.
The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

Rathor, G.S.; Swain, B. Advancements in Fish Vaccination: Current Innovations and Future Horizons in Aquaculture Health Management. Appl. Sci. 2024 , 14 , 5672. https://doi.org/10.3390/app14135672

Rathor GS, Swain B. Advancements in Fish Vaccination: Current Innovations and Future Horizons in Aquaculture Health Management. Applied Sciences . 2024; 14(13):5672. https://doi.org/10.3390/app14135672

Rathor, Garima S., and Banikalyan Swain. 2024. "Advancements in Fish Vaccination: Current Innovations and Future Horizons in Aquaculture Health Management" Applied Sciences 14, no. 13: 5672. https://doi.org/10.3390/app14135672

Article Metrics

Article access statistics, further information, mdpi initiatives, follow mdpi.

MDPI

Subscribe to receive issue release notifications and newsletters from MDPI journals

IMAGES

  1. 🏷️ Formulation of hypothesis in research. How to Write a Strong

    formulation of hypothesis examples

  2. Hypothesis

    formulation of hypothesis examples

  3. 🏷️ Formulation of hypothesis in research. How to Write a Strong

    formulation of hypothesis examples

  4. PPT

    formulation of hypothesis examples

  5. How to Formulate a Hypothesis for an Experiment

    formulation of hypothesis examples

  6. How to Write a Hypothesis

    formulation of hypothesis examples

VIDEO

  1. Concept of Hypothesis in Hindi || Research Hypothesis || #ugcnetphysicaleducation #ntaugcnet

  2. Formulation and Testing of Hypothesis

  3. Research Methodology Video 4

  4. Formulation of hypothesis |Biological method

  5. Hypothesis Formulation

  6. 9

COMMENTS

  1. How to Write a Strong Hypothesis

    Developing a hypothesis (with example) Step 1. Ask a question. Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project. Example: Research question.

  2. What is a Research Hypothesis: How to Write it, Types, and Examples

    Here are some good research hypothesis examples: "The use of a specific type of therapy will lead to a reduction in symptoms of depression in individuals with a history of major depressive disorder.". "Providing educational interventions on healthy eating habits will result in weight loss in overweight individuals.".

  3. PDF 1. Formulation of Research Hypothesis with student samples

    Your hypothesis is what you propose to "prove" by your research. As a result of your research, you will arrive at a conclusion, a theory, or understanding that will be useful or applicable beyond the research itself. 3. Avoid judgmental words in your hypothesis. Value judgments are subjective and are not appropriate for a hypothesis.

  4. How to Write a Strong Hypothesis

    Step 5: Phrase your hypothesis in three ways. To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable. If a first-year student starts attending more lectures, then their exam scores will improve.

  5. Hypothesis: Definition, Examples, and Types

    A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process. Consider a study designed to examine the relationship between sleep deprivation and test ...

  6. Research Hypothesis: Definition, Types, Examples and Quick Tips

    3. Simple hypothesis. A simple hypothesis is a statement made to reflect the relation between exactly two variables. One independent and one dependent. Consider the example, "Smoking is a prominent cause of lung cancer." The dependent variable, lung cancer, is dependent on the independent variable, smoking. 4.

  7. What is a Hypothesis

    The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. ... and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research. Characteristics of Hypothesis.

  8. How to Write a Hypothesis w/ Strong Examples

    The formulation of a hypothesis is a big step in the scientific method, as it defines the focus and direction of the research. A lot of time is often spent simply on developing a good hypothesis. ... For example, a hypothesis for the research question stated above might be: "If sunflower plants are watered with varying amounts of water, then ...

  9. Formulating Strong Hypotheses

    Formulating Strong Hypotheses. Before you write your research hypothesis, make sure to do some reading in your area of interest; good resources will include scholarly papers, articles, books, and other academic research. Because your research hypothesis will be a specific, testable prediction about what you expect to happen in a study, you will ...

  10. Scientific hypothesis

    The formulation and testing of a hypothesis is part of the scientific method, the approach scientists use when attempting to understand and test ideas about natural phenomena. The generation of a hypothesis frequently is described as a creative process and is based on existing scientific knowledge, intuition , or experience.

  11. Hypothesis Examples

    Here are some research hypothesis examples: If you leave the lights on, then it takes longer for people to fall asleep. If you refrigerate apples, they last longer before going bad. If you keep the curtains closed, then you need less electricity to heat or cool the house (the electric bill is lower). If you leave a bucket of water uncovered ...

  12. Hypothesis Testing

    Present the findings in your results and discussion section. Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps. Table of contents. Step 1: State your null and alternate hypothesis. Step 2: Collect data. Step 3: Perform a statistical test.

  13. How to Write a Research Hypothesis: Good & Bad Examples

    But considering that the formulation and testing of hypotheses is an integral part of the scientific method, it is good to be aware of the concepts underlying this approach. ... This is then called a one-tailed hypothesis. Another example for a directional one-tailed alternative hypothesis would be that . H1: Attending private classes before ...

  14. Exploring Research Question and Hypothesis Examples: A Comprehensive G

    Analyzing Hypothesis Examples Across Fields Case Studies in Psychology. ... When you embark on hypothesis formulation, understanding the distinction between quantitative and qualitative research methodologies is crucial. Quantitative research focuses on numerical data and statistical analysis, ideal for hypotheses that require measurable ...

  15. Research Hypothesis In Psychology: Types, & Examples

    Examples. A research hypothesis, in its plural form "hypotheses," is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

  16. Formulation of Hypotheses: Definition, Types & Example

    The hypothesis is a predictive, testable statement predicting the outcome and the results the researcher expects to find. The hypothesis provides a summary of what direction, if any, is taken to investigate a theory. In scientific research, there is a criterion that hypotheses need to be met to be regarded as acceptable.

  17. Formulation of Hypothesis & Examples

    Formulation of Hypothesis & Examples Lesson; Transcript Heather Saigo, Devin Kowalczyk Author. Heather Saigo. Heather is a science educator with a bachelor's degree in biology and a master's ...

  18. What a Hypothesis Is and How to Formulate One

    A hypothesis is a prediction of what will be found at the outcome of a research project and is typically focused on the relationship between two different variables studied in the research. It is usually based on both theoretical expectations about how things work and already existing scientific evidence. Within social science, a hypothesis can ...

  19. (PDF) FORMULATING AND TESTING HYPOTHESIS

    Example 1 is a hypothesis for a nonexperimental study. ... Personal experience: Not only do culture, science and analogy, among others, affect the formulation . of hypothese s.

  20. Formulation of Hypothesis & Examples

    A null hypothesis is a statement of no relationship between the independent variable and dependent variable. Read Formulation of Hypothesis & Examples Lesson Recommended for You

  21. Hypothesis

    Alternative Hypothesis (H1): Example: There is a significant effect of caffeine consumption on cognitive performance. Directional Hypothesis: Example: Caffeine consumption will improve cognitive performance. Non-Directional Hypothesis: Example: There is a relationship between caffeine consumption and cognitive performance. 5. Refining the ...

  22. Formulation of Hypotheses: Definition, Types & Example

    The hypothesis is a predictive, testable statement predicting the outcome and the results the researcher expects to find. The hypothesis provides a summary of what direction, if any, is taken to investigate a theory. In scientific research, there is a criterion that hypotheses need to be met to be regarded as acceptable.

  23. Advancements in Fish Vaccination: Current Innovations and Future ...

    Aquaculture is rapidly becoming one of the pivotal sectors in the farming economy, driven by the increasing demand for high-quality animal protein at an affordable cost, especially with the escalating human population. However, the expansion of high-density fish populations also brings forth a challenge—the rapid transmission and spread of infectious disease agents among them. To combat this ...